摘要
马传染性贫血病(简称马传贫)是严重危害马、骡、驴的一种病毒性传染病,在急性爆发时,往往造成大批死亡。患过病的动物体内病毒长期不消失,持续感染,反复发病,成为传染源。控制和消灭家畜传染病的根本措施之一,是提供主动免疫的疫苗。但患过马传贫病的马能否获得免疫以及本病能否进行人工免疫,是多年来学者们所重视和争论的问题。
Having successfully developed donkey leucocyte cultures for the growth and propagation of the virus of Equine Infectious Anaemia(EIA),we have made the EIA virus propagated and serially passaged through this caltured cell since 1967.Withthe increase of the number of passages in this cultured cell,the pathogenicity of the virus for the culured cells could be gradually enhanced the titre up to a level of 7.0 TCID_(50) per ml or higher.On the contrary,the pathogenicity of the passaged virus for the horses and donkeys was markedly reduced.Virus from the 100th passage onwards was seldom produce the disease upon inoculated horses and donkeys.The virus was also passaged serially in horses and donkeys without any signs of reversion of its attenuated virulence.Healthy horses contacting with those inoculated were free from infection.It was demonstrated that,after the attenuated virus propagated in the inoculated bodies of horses or donkeys,immune responses were induced and conferred on the vaccinated animals substantial protection against the infection.When these vaccinated animals were challenged with a virulent virus,the protection rate obtained in horses was 79 per cent and nearly 100 per cent in donkeys.However,the immunity was found to develop rather slowly,taking about 6 months to become strong enough to resist infection.
作者
沈荣显
徐振东
何云生
张盛兴
Shen Rong-xian;Xu Zhen-dong;He Yun-sheng;Zhang Sheng-xing(Harbin Veteinary Institute of the Chinese Academy of Agricultural Sciences)
出处
《中国农业科学》
1979年第4期1-15,共15页
Scientia Agricultura Sinica
