摘要
我国花生育种大多是采用品种间杂交的方法,由于遗传基础狭窄,育成的品种往往适应性不广,抗病虫能力较差。因此,利用花生野生种质资源进行育种,已引起广泛重视。近年来,我国从国外引进花生野生种质材料20多份,其中绝大多数是二倍体(2n=20),与栽培种杂交只能产生不育的三倍体(2n=30)。要利用花生野生种质资源,首先必须解决三倍体杂种染色体加倍问题。我们自1982年至1983年对这项技术进行了一些研究,现将初步结果报道如下。
The peanut interspecific triploid Ffrom 4 varieties type of Arachis hypogaea L.crossing with diploid wild species A.cardenasii was treated with colchicine and DMSO to study the technique of somatic doubling.It was shown that the treatment of three-day seedling’s apical bud with 0.2%—0.4%colchicine plus 3%DMSO under 25—30℃from 8 a.m.to 4 p.m.by dropping methed was most effective.The somatic doubling-rate reached about 70%,and even 100%,while the natural somatic doubling was only 0—9.8%,the average was 5.7%.
作者
申馥玉
王在序
甘信民
Shen Fuyu;Wang Zaixu;Gan Xinmin(Shandong Institute of Peanut, Laixi, Shandong Province)
出处
《中国农业科学》
1984年第4期21-25,共5页
Scientia Agricultura Sinica
