摘要
该文以米曲霉为研究对象,通过单因素试验和正交试验,确定了米曲霉固体培养的最佳条件。试验结果表明,最佳组合为玉米粉添加量为20%、大豆蛋白添加量为10%、农作物秸秆+麸皮添加量为65%、培养温度为34~36℃、培养时间为72 h、培养基起始含水量控制在55%,在此条件下米曲霉的蛋白酶酶活力达到13400 U/g、孢子数为130.5亿个/g,发芽率为92%。采用优化后的种曲进行圆盘制曲培养,优化组的蛋白酶酶活力平均值为3376 U/g、孢子数平均值为2.6亿个/g,较对照组分别提升了18.2%和30%。
This paper takes Aspergillus oryzae as the research object,and determines the optimal conditions for solid-state cultivation of Aspergillus oryzae through single factor experiment and orthogonal experiment.The results showed that the optimal combination was 20%corn flour adding amount,10%soybean protein adding amount,65%crop straw+bran adding amount,34~36℃culture temperature,72 h culture time,and 55%initial water content of culture medium.Under these conditions,the protease activity of Aspergillus oryzae reached 13400 U/g,the number of spores was 13.05 billion/g,and the germination rate was 92%.The optimized seed koji was used for disc culture,and the average protease activity and spore number of the optimized group were 3376 U/g and 260 million/g,which increased by 18.2%and 30%,respectively,compared to the control group.
作者
李艳军
赖理智
李荔
LI Yanjun;LAI Lizhi;LI Li(Foshan Haitian(Gaoming)Flavoring&Food Co.,Ltd.,Foshan 528500,China)
出处
《食品与发酵科技》
2025年第6期47-55,共9页
Food and Fermentation Science & Technology
基金
广东省调味食品生物发酵先进技术企业重点实验室(佛山市海天(高明)调味食品有限公司)开放基金(2017B030302002)。
关键词
米曲霉
碳源
氮源
正交试验
Aspergillus oryzae
carbon sources
nitrogen sources
orthogonal experiment
