摘要
为解析叶黄素(Lutein,LUT)和矢车菊素-3-O-葡萄糖苷(Cyanidin-3-O-glucoside,C3G)单独及联合使用使肝脏免受氧化损伤的作用机制,本研究以四氯化碳(CCl_(4))诱导构建小鼠肝脏氧化损伤模型,比较LUT、C3G及LC(LUT+C3G)处理对小鼠肝脏氧化标志物、Nrf2通路调控基因表达水平及肠道菌群结构的影响。结果表明,与CCl_(4)处理相比,LUT、C3G、LC处理均能提高小鼠肝脏中总超氧化物歧化酶(T-SOD)活性、过氧化氢酶(CAT)活性、谷胱甘肽(GSH)含量和总抗氧化能力(T-AOC),有效抑制丙二醛(MDA)的产生,减轻小鼠肝脏氧化损伤。其中,LC处理小鼠肝脏GSH含量、T-AOC分别比LUT处理增加50.66%、39.23%,MDA含量降低41.04%。与CCl_(4)处理相比,LUT、C3G、LC处理均能提高Nrf2、NQO1和HO-1基因的表达水平,降低Keap1基因的表达水平,其中,LC处理Nrf2、NQO1、HO-1基因相对表达量分别比LUT处理上调57.40%、46.50%、42.77%,Keap1基因相对表达量下调40.88%。LUT、C3G、LC处理能显著增加短链脂肪酸(SCFA)的产生,并增加肠道理研菌科(Rikenellaceae)和阿克曼氏菌科(Akkermansiaceae)细菌的相对丰度,而抗生素预处理则能干扰LUT和C3G对肠道菌群的调控,降低肠道毛螺菌科(Lachnospiraceae)和类杆菌科(Bacteroidaceae)细菌的相对丰度。综上,LUT和C3G联合使用能增强小鼠肝脏抗氧化酶活性、调控Nrf2信号通路基因表达水平以及改善肠道菌群结构。
To analyze the mechanism of action of lutein(LUT)and cyanidin-3-O-glucoside(C3G)alone and in combination on liver oxidative damage,this study constructed a mouse liver oxidative damage model induced by carbon tetrachloride(CCl_(4)),and compared the effects of LUT,C3G and LC(LUT+C3G)treatments on liver oxidative markers,Nrf2 pathway regulatory gene expression levels and gut microbiota structure in mice.The results showed that compared with CCl_(4)treatment,LUT,C3G and LC treatments increased the activities of total superoxide dismutase(T-SOD)and catalase(CAT),elevated glutathione(GSH)content and total antioxidant capacity(T-AOC)in the liver of mice,and effectively inhibited the production of malondialdehyde(MDA),thereby alleviating hepatic oxidative damage in mice.Compared with the LUT-treated group,the GSH content and T-AOC in the liver of LC-treated mice increased by 50.66%and 39.23%,respectively,and the MDA content decreased by 41.04%.Compared with CCl_(4)treatment,LUT,C3G and LC treatments could increase the expression levels of Nrf2,NQO1 and HO-1 genes and decrease the expression level of Keap1 gene.The relative expression levels of Nrf2,NQO1 and HO-1 genes in LC treatment were 57.40%,46.50%and 42.77%higher than those in LUT treatment,while the relative expression level of Keap1 gene was down-regulated by 40.88%.LUT,C3G and LC treatments significantly increased the production of short-chain fatty acids(SCFA)and the relative abundance of Rikenellaceae and Akkermansia bacteria,while antibiotic pretreatment could interfere with the regulation of LUT and C3G on gut microbiota and reduce the relative abundance of Lachnospiraceae and Bacteroidaceae bacteria.In summary,the combined use of LUT and C3G can enhance the activty of antioxidant enzymes in the liver of mice,regulate the expression levels of Nrf2 pathway regulatory genes and improve the gut microbiota structure of mice.
作者
陆风芹
李艳
李大婧
周存山
徐亚元
何伟伟
戴竹青
冯蕾
连运河
张钟元
LU Fengqin;LI Yan;LI Dajing;ZHOU Cunshan;XU Yayuan;HE Weiwei;DAI Zhuqing;FENG Lei;LIAN Yunhe;ZHANG Zhongyuan(School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China;Institute of Agricultural Products Processing,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Chenguang Biotechnology Group Co.,Ltd.,Shijiazhuang 050000,China)
出处
《江苏农业学报》
北大核心
2025年第11期2242-2256,共15页
Jiangsu Journal of Agricultural Sciences
基金
江苏省科技计划专项资金项目(BN2023060)。
