摘要
目的:研究电化学高级氧化技术(EAOP)在口腔牙齿漂白中的效率、生物安全性问题,并提出初步应用方式。方法:以靛蓝胭脂红和咖啡为指示剂,进行EAOP的效果验证;高电阻计测量牙齿电阻,验证工作电压安全性;收集完整智齿20颗,染色后通过离漂白实验验证漂白效率;对漂白后牙齿进行扫描电镜检测、硬度检测、细菌黏附实验,评估牙齿表面损伤情况;细胞共培养验证其细胞毒性;制备漂白牙托初样,验证耐用性。结果:EAOP可在8 V的工作电压下,可以在10min内实现靛蓝胭脂红的漂白,在90 min内实现咖啡漂白。牙齿窝沟处电阻为(3.4±1.2)MΩ,理论计算电流小于3μA。EAOP牙齿漂白效率略低于传统诊室漂白,高于家庭漂白。相比于传统漂白法,扫描电镜检测发现EAOP对牙齿表面的损伤作用更小;漂白前后牙齿表面硬度变化无统计学差异(P=0.912);细菌黏附实验表明,EAOP法可以减少约60%的细菌黏附(P<0.001)。细胞毒性实验表明,EAOP电解液对L929细胞无明显毒性。耐用性实验表明,经过20 h使用后依旧具有良好的漂白性能。结论:EAOP漂白具有优异的牙齿漂白效果,对牙齿损伤作用小,安全性高,相关漂白装置拥有良好的耐用性。
Objective:To study the efficiency and biosafety of Electrochemical advanced oxidation processes(EAOP)in dental bleaching,and conduct preliminary application.Methods:Indigo carmine and coffee were used as the indicator to assess the efficacy of EAOP.High resistance meter was used to measure the resistance of the tooth to verify the safety of the working voltage.Twenty wisdom teeth after tooth extraction were collected,dyed and bleached in vitro to verify the bleaching efficiency.Subsequently,the bleached teeth were examined by scanning electron microscopy,hardness testing,and bacterial adhesion experiments to assess surface damage.To determine its cytotoxicity,cells were co-cultured with electrolyte.Initial samples of bleaching tray was prepared,and its durability were verified.Results:The EAOP could bleach indigo carmine within 10 min and coffee within 90 min at an operating voltage of 8 V.The resistance at the groove of the tooth socket was(3.4±1.2)MΩ,and the theoretical calculated current was less than 3μA.The efficiency of EAOP tooth bleaching was slightly lower than that of traditional office bleaching and higher than that of home bleaching.Compared with the traditional bleaching method,scanning electron microscopy showed that EAOP had less demineralization effect on tooth surface.The tooth hardness before and after bleaching had no statistical difference(P=0.912).The bacterial adhesion test after tooth bleaching showed that EAOP method could reduce about 60%bacterial adhesion(P<0.001).The cytotoxicity test showed that EAOP electrolyte had no obvious toxic effect.The durability test shows that the bleached denture still has good bleaching effect after 20 h of use.Conclusion:Compared with the traditional bleaching method,EAOP bleaching had excellent tooth bleaching effect,little effect on tooth damage,high safety,and the related bleaching devices had good durability.
作者
范文杰
张佳琪
占佳琪
傅含霓
何先哲
许琳
黄希露
胡丽
FAN Wenjie;ZHANG Jiaqi;ZHAN Jiaqi;FU Hanni;HE Xianzhe;XU Lin;HUANG Xilu;HU Li(Department of Stomatology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;School of Stomatology,Tongji Medical College,Huazhong University of Science and Technology)
出处
《实用口腔医学杂志》
北大核心
2025年第6期756-762,共7页
Journal of Practical Stomatology
基金
武汉强磁场学科交叉基金(编号:WHMFC202207)
中国牙病防治基金会爱笑少年项目(编号:A2023-009)。
关键词
牙齿漂白
电化学高级氧化技术
牙釉质
安全性
Tooth bleaching
Electrochemical advanced oxidation processes
Dental enamel
Safety
