摘要
[目的]探讨生脉胶囊对小鼠化疗性肝损伤的保护作用和分子机制。[方法]比较对照组、化疗组和化疗联合生脉胶囊治疗组小鼠的体质量、肝质量和肝脏指数,检测血清丙氨酸氨基转移酶(ALT)和天冬氨酸氨基转移酶(AST)活性,并对肝脏组织病理形态学进行检查,应用核磁共振氢谱(1H-NMR)代谢组学分析肝脏差异代谢物,探讨生脉胶囊减轻化疗性肝损伤的可能机制。[结果]化疗联合生脉胶囊显著逆转单纯化疗导致的小鼠体质量、肝质量和肝脏指数的降低,下调血清AST活性,减轻肝组织水肿和嗜酸性样变。代谢组学分析表明,化疗联合生脉胶囊治疗了上调牛磺酸、谷氨酰胺和褪黑素等代谢物的水平,这些差异代谢物主要富集在糖代谢和氨基酸代谢等通路。[结论]生脉胶囊能够通过调节糖代谢和氨基酸代谢减轻小鼠化疗性肝损伤。
[Objective]To explore the protective effect and molecular mechanism of Shengmai Capsules on chemotherapy-induced liver injury of mice.[Methods]The body weight,liver weight and liver index of mice in the control group,chemotherapy group and chemotherapy combined with Shengmai Capsules group were recorded and compared.Serum activities of alanine aminotransferase(ALT)and aspartate aminotransferase(AST)were detected and liver tissues were examined by pathomorphology.Proton nuclear magnetic resonance(1H-NMR)metabolomics were performed to analyze differential metabolites in liver and to investigate the possible role and mechanism of Shengmai Capsules in alleviating chemotherapy-induced liver injury.[Results]chemotherapy combined with Shengmai Capsules treatment significantly reversed the decrease in body weight,liver weight and liver index caused by chemotherapy,and downregulated serum AST activity,as well alleviated edema and eosinophilic changes in liver tissues.Metabolomic analysis showed that combined treatment with chemotherapy and Shengmai Capsule elevates the levels of taurine,glutamine,and melatonin in mice liver.These metabolites were mainly enriched in glucose metabolism pathway and amino acid metabolism pathways.[Conclusion]Shengmai Capsules can alleviate chemotherapy-induced liver injury in mice by regulating glucose metabolism and amino acid metabolism pathways.
作者
巩祖妍
李兆栋
李典
赵舒武
苗琳
王丽明
韩立峰
阎皓
许彦杰
彭雁飞
GONG Zuyan;LI Zhaodong;LI Dian;ZHAO Shuwu;MIAO Lin;WANG Liming;HAN Lifeng;YAN Hao;XU Yanjie;PENG Yanfei(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Nankai University Affiliated People’s Hospital,Tianjin 300121,China;The Third Central Hospital of Tianjin,Tianjin 300170,China)
出处
《天津中医药》
2025年第12期1591-1597,共7页
Tianjin Journal of Traditional Chinese Medicine
基金
天津市卫生健康委中医、中西医结合科研课题重点项目(2023034)。
关键词
化疗性肝损伤
生脉胶囊
核磁共振
代谢组学
chemotherapy-induced liver injury
Shengmai Capsules
nuclear magnetic resonance
metabolomics
