摘要
目的:探讨钙激活氯通道蛋白1(ANO1)和E-钙黏蛋白(E-cadherin)在结直肠癌(CRC)组织中的表达及其与CRC患者临床病理特征的关系,并阐明其临床意义。方法:收集原发性CRC患者的手术组织样本77例。采用免疫组织化学(IHC)法检测CRC组织中ANO1和E-cadherin蛋白表达情况,采用Spearman相关分析法分析二者表达的相关性,采用PCR荧光探针法检测CRC组织中KRAS/NRAS突变情况,采用卡方检验或Fisher确切概率法分析ANO1和E-cadherin表达与CRC患者临床病理特征的关系。结果:IHC法检测,CRC组织中ANO1阳性表达率为24.7%,E-cadherin阳性表达率为63.6%。Spearman法分析,CRC组织中ANO1与E-cadherin表达呈正相关关系(r=0.458,P<0.05)。肿瘤部位在结肠的CRC患者癌组织中ANO1阳性表达率明显高于肿瘤部位在直肠的患者(χ^(2)=5.499,P=0.019);TNM分期Ⅰ-Ⅱ期CRC患者癌组织中ANO1阳性表达率明显高于Ⅲ-Ⅳ期的患者(χ^(2)=4.774,P=0.029);无淋巴结转移的CRC患者ANO1阳性表达率明显高于有淋巴结转移的患者(P=0.034)。T1-T3期CRC患者E-cadherin阳性表达率明显高于T4期的CRC患者(P=0.024)。p53阴性、Ki-67<90%、PMS2阴性和KRAS突变型的CRC患者癌组织中ANO1阳性表达率明显高于p53阳性、Ki-67≥90%、 PMS2阳性和KRAS野生型的CRC患者(P=0.031, P=0.036,P=0.048, P=0.028)。结论:患者原发性CRC组织中ANO1与E-cadherin表达呈正相关关系,ANO1及E-cadherin与患者多个临床病理特征存在关联性,ANO1和E-cadherin可能参与CRC的发生和进展过程。
Objective:To explore the expressions of calcium-activated chloride channel protein 1(ANO1)and E-cadherin in colorectal cancer(CRC)tissue and their relationships with the clinicopathological characteristics of the CRC patients,and to clarify their clinical significances.Methods:The surgical tissue specimens from 77 patients with primary CRC were collected.Immunohistochemistry(IHC)method was used to detect the protein expressions of ANO1 and E-cadherin in CRC tissue;Spearman correlation analysis method was used to analyze the correlation between their expressions;PCR fluorescence probe method was used to detect the KRAS/NRAS mutations in CRC tissue;chi-square test or Fisher’s exact probability method was used to analyze the relationships between the expressions of ANO1 and E-cadherin and clinicopathological characteristics of the CRC patients.Results:The IHC results showed that the positive expression rate of ANO1 in CRC tissue was 24.7%,and the positive expression rate of E-cadherin was 63.6%.The Spearman analysis results showed that the expressions of ANO1 and E-cadherin in CRC tissue were positively correlated(r=0.458,P<0.05).Compared with the patients with tumor located in rectum,the positive expression rate of ANO1 in cancer tissue of the CRC patients with tumor located in colon was significantly increased(χ^(2)=5.499,P=0.019);compared with the patients with TNM stageⅢ-Ⅳ,the positive expression rate of ANO1 in cancer tissue of the CRC patients with TNM stageⅠ-Ⅱwas significantly increased(χ^(2)=4.774,P=0.029);compared with the patients with lymphnode metastasis,the positive expression rate of ANO1 in the CRC patients without lymphnode metastasis was significantly increased(P=0.034).Compared with the CRC patients with T4 stage,the positive expression rate of E-cadherin in the CRC patients with T1-T3 stage was significantly increased(P=0.024).Compared with the CRC patients with p53 positive,Ki-67≥90%,PMS2 positive and KRAS wild-type,the positive expression rate of ANO1 in cancer tissue of the CRC patients with p53 negative,Ki-67<90%,PMS2 negative and KRAS mutant was significantly increased(P=0.031,P=0.036,P=0.048,P=0.028).Conclusion:The expressions of ANO1 and E-cadherin in primary CRC tissue of the patients are positively correlated;ANO1 and E-cadherin are associated with multiple clinicopathological characteristics of the patients;ANO1 and E-cadherin may be involved in the occurrence and progression of CRC.
作者
吴飞
杨麒巍
李馨
赵林先
隋玉杰
WU Fei;YANG Qiwei;LI Xin;ZHAO Linxian;SUI Yujie(Department of Gynecology and Obstetrics,Second Hospital,Jilin University,Changchun 130041,China;Key Laboratory for Molecular and Chemical Genetics of Critical Human Diseases of Jilin Province,Medical Research Center,Second Hospital,Jilin University,Changchun 130041,China;Department of General Surgery,Second Hospital,Jilin University,Changchun 130041,China)
出处
《吉林大学学报(医学版)》
北大核心
2025年第6期1638-1645,共8页
Journal of Jilin University:Medicine Edition
基金
吉林省科技厅自然科学基金项目(YDZJ202201ZYTS026)
吉林省教育厅科技项目(JJKH20231220KJ)。
