摘要
为探究香菇等4种食用菌混合制备的多糖提取物对小鼠理化指标和肠道菌群的调节作用,本研究采用超声辅助水提法制备复合食用菌多糖提取物,然后将制备的提取物补充给昆明小鼠并持续灌胃35 d,通过测定小鼠体重、脏器指数、理化指标、炎症因子及肠道菌群组成变化来评价其效果。结果表明,与空白组小鼠相比,复合多糖的摄入对小鼠体重和肾脏、脾脏指数未产生显著影响(P>0.05),但降低了小鼠血清和尿液中尿酸、尿素氮和肌酐的水平,并抑制了体内黄嘌呤氧化酶的活性;复合多糖摄入降低了血清中炎症因子白介素-6和肿瘤坏死因子-α以及D-乳酸和二胺氧化酶的水平,显著改善了肠道屏障功能;复合多糖的摄入提高了小鼠肝脏中抗氧化酶(总抗氧化能力、过氧化氢酶和超氧化物歧化酶)的活性,降低了丙二醛含量,改善了体内氧化应激反应。16S rRNA测序结果表明,复合多糖补充较空白组小鼠显著上调了乳酸杆菌科(Lactobacillaceae)及鼠乳杆菌(Lactobacillus murinus)等益生菌的相对丰度(P<0.05)。综上,4种食用菌混合制备的多糖提取物不仅能增强小鼠的免疫能力,还能调节肠道菌群。本研究为食用菌多糖用作膳食补充剂提供了理论依据。
To investigate the regulatory effects of polysaccharides extracts derived from a blend of four edible mushrooms on physiological parameters and gut microbiota in mice,mixed mushroom polysaccharides extracts using ultrasonic-assisted water extraction were prepared in this study.The extracts were administered to Kunming mice via oral gavage for 35 consecutive days.The therapeutic effects were evaluated through monitoring body weight,organ indices,biochemical parameters,inflammatory cytokines,and gut microbiota composition.Results demonstrated that mixed polysaccharides supplementation had no significant effects on body weight,renal and spleen indices(P>0.05).However,mixed polysaccharides supplementation reduced serum and urinary levels of uric acid,urea nitrogen,and creatinine,while suppressing xanthine oxidase activity.Concurrently,mixed polysaccharides intake decreased serum levels of interleukin-6 and tumor necrosis factor-α and intestinal permeability markers(D-lactate and diamine oxidase),indicating enhanced gut barrier integrity.Compared with the control group mice,mixed polysaccharides administration significantly elevated hepatic antioxidant enzyme activities(total antioxidant capacity,catalase,and superoxide dismutase)while reducing malondialdehyde content,ameliorating systemic oxidative stress.16S rRNA sequencing revealed that mixed polysaccharides supplementation notably increased the relative abundance of beneficial bacteria,particularly Lactobacillaceae,with significant proliferation of Lactobacillus murinus(P<0.05)when compared with the control group mice.In summary,the polysaccharide extracts prepared by mixing four kinds of edible mushrooms not only enhanced the immunity of mice,but also regulated gut microbiota.The findings of this study provide theoretical foundations for developing mushroom polysaccharides as health-promoting dietary supplements.
作者
张琪
殷朝敏
范秀芝
史德芳
李耀东
蒋文重
陈盛
高虹
ZHANG Qi;YIN Chaomin;FAN Xiuzhi;SHI Defang;LI Yaodong;JIANG Wenzhong;CHEN Sheng;GAO Hong(Institute of Agro-Products Processing and Nuclear Agricultural Technology,Hubei Academy of Agricultural Sciences,Wuhan,Hubei 430064;College of Life Science,Yangtze University,Jingzhou,Hubei 434025;Fuheshou(Wuhan)Health Management Co.,LTD.,Wuhan,Hubei 430024;Hubei Haowei Technology Co.,LTD.,Zhongxiang,Hubei 448000;Hubei Xianggu Mushroom Industrial Technology Research Institute,Suizhou,Hubei 441309)
出处
《核农学报》
北大核心
2026年第1期127-137,共11页
Journal of Nuclear Agricultural Sciences
基金
国家自然科学基金(32072229)
湖北省技术研发重大项目(2024BBB028)
国家重点研发计划课题(2024YFD2100801)
湖北省食用菌产业技术体系项目(2023HBSTX4-09)。
关键词
多糖提取物
尿酸
炎症
肠道菌群
polysaccharides extracts
uric acid
inflammation
gut microbiota
