摘要
旨在探究不同性别麋鹿(Elaphurus davidianus)在发情期肠道微生物的群落结构和功能特征上的差异。本研究采用宏基因组测序技术,对6只发情期麋鹿(3只雄性和3只雌性)的肠道微生物进行分析,深入解析不同性别麋鹿发情期肠道微生物的组成和功能。结果:在发情期麋鹿粪便中,共鉴定出20个门、20个属和20个种,且雄性和雌性麋鹿的肠道微生物在门、属和种水平上存在显著差异;雄性麋鹿肠道中鲁格利亚菌属(Lugdunibacter)、假单胞菌属(Pseudomonas)等5种菌属丰度显著高于雌性,而雌性麋鹿肠道中食藻属(Algoriphagu)、链球菌属(Streptococ-cus)等3种菌属丰度显著高于雄性;发情期雄性和雌性麋鹿肠道微生物的多样性指数和均匀度指数无显著差异;功能预测分析进一步揭示了肠道微生物在代谢功能上的关键作用,特别是碳水化合物代谢方面;雄性麋鹿的肠道微生物功能主要倾向于糖基转移反应,而雌性麋鹿则偏向于糖代谢酶的富集。综上,本研究为理解麋鹿繁殖机制提供了新的视角,并为通过调节肠道微生物来改善麋鹿的繁殖健康状况提供了理论依据。
This study was to investigate the differences in the community structure and functional characteristics of intestinal microbiota be-tween males and females of Elaphurus davidianus during estrus.Metagenomic sequencing technology was used to analyze the gut microbiota of six elk(three males and three females)during estrus,investigating the variations in gut microbial composition and function between sexes.The results were that 20 phyla,20 species,and 20 genera were identified across the samples,revealing significant differences in gut microbi-ota at the phylum,genus,and species levels between male and female elks.Specifically,the abundance of Lugdunibacter and Pseudomonas increased in the gut of the male elks,while the abundance of Algoriphagu and Streptococcus increased in the gut of the female elks.Notably,the diversity index and evenness index of gut microbes did not differ significantly between male and female elks in estrous.Functional predic-tion analysis further highlighted the importance of gut microbes in metabolic functions,particularly carbohydrate metabolism.The microbial functions in the gut of the male elks were predominantly involved in glycosyl transfer reactions,whereas the female elk exhibited an enrich-ment of enzymes related to glucose metabolism.These findings provided new insights into the mechanisms of elk reproduction and offered a ra-tionale for improving elk reproductive health through the regulation of gut microbiota.
作者
李婷
袁红平
张艺川
乔征磊
原宝东
LI Ting;YUAN Hongping;ZHANG Yichuan;QIAO Zhenglei;YUAN Baodong(College of Life Science and Technology,Mudanjiang Normal University,Mudanjiang 157011,China;Yancheng Milu Deer Research Institute,Yancheng 224136,China;College of Agriculture and Biology,Liaocheng University,Liaocheng 252000,China)
出处
《畜牧与兽医》
北大核心
2025年第12期34-42,共9页
Animal Husbandry & Veterinary Medicine
基金
山东省自然科学基金项目(ZR2023MC016)
江苏省林业科技创新与推广项目。
关键词
麋鹿
粪便微生物组
发情期
宏基因组学
功能预测
Elaphurus davidianus
fecal microbiome
estrus
metagenomics
functional prediction
