摘要
中华绒螯蟹的精子属无鞭毛精子。扫描电镜观察表明,这类精子呈不规则扁球形,前端有一光滑的圆面,圆面四周是凹陷的沟环,沟环后的精子表面凹凸不平,并伸出约20条辐射臂。透射电镜揭示了这类精子是由球形的顶体、杯状的核杯及辐射臂所组成,其中顶体形态较为复杂,包含多种亚显微结构。细胞化学反应证明了核杯为Feulgen阳性,顶体为PAS阳性。
The sperm of the Chinese mitten-handed crab(Eriocheir sinensis)was studied with Scanning Electron Microscope(SEM),Transmission Electron Microscope(TEM)and cytochemi-cal methods.The sperm of the crab belongs to a flagellate sperm.SEM observation shows that the sperm has a spherical body,the anterior part of which appears smooth,and the other part is uneven,from which radiate a number of radial arms.Between the two parts there is a ditch ring,in which some papillae can be found.TEM reveals that the sperm is composed of acrosome,nuclear cup and radial arms.Acrosome is a complicated globular structure and shows PAS positive,the centre of which is a cylindrical acrosomal tubule,the front of which is an apical cap.Around the acrosomal tubule is the acrosomal vesicle,which can be divided into a fibrous layer,a middle layer and some lamellar structures.The centriole is located near the base of the acrosomal tubule.Except the area of the apical cap,the acrosome is surrounded by nuclear cup,which shows Feulgen positive.Some mitochondrias can be seen distinctively surrounded by the nuclear envelope near the edge of the nuclear cup.There are about twenty radial arms formed by nuclear processes.
作者
堵南山
赖伟
薛鲁征
Du Nanshan;Lai Wei;Xue Luzhen(East China Normal University,Shanghai)
出处
《海洋与湖沼》
1987年第2期119-125,216,共8页
Oceanologia Et Limnologia Sinica
