摘要
【目的】筛选盐胁迫下沙芥抗逆种质资源转录组的关键基因,为解析沙芥耐盐抗逆生理研究奠定基础,并为沙芥耐盐分子育种提供参考。【方法】测定6叶1心期沙芥幼苗在0、50、100、150、200 mmol/L NaCl处理8 d时的叶片鲜重比,并以鲜重比50%左右的盐浓度为转录组处理浓度,分别在0、3、12 h取样进行转录组测序。【结果】通过测定叶鲜重比,确定沙芥的盐阈值为100 mmol/L;与0 h相比,3和12 h的转录组数据分别鉴定出10006和14117个差异表达基因;转录因子共筛选出4个上调表达的基因。植物激素信号转导过程中,油菜素甾醇(BR)上调基因最多;苯丙烷类代谢途径共筛选出3个上调的关键酶,类黄酮生物合成途径共筛选出4个上调的关键酶。【结论】转录因子、植物激素信号转导过程、苯丙烷类代谢途径和类黄酮生物合成途径中的上调基因可能在沙芥受到盐胁迫时起关键作用。
【Objective】To screen key genes in the transcriptome of stress-tolerant germplasm resources of Pugionium cornutum under salt stress,thereby laying a foundation for elucidating the physiological research of salt-tolerance and stress resistance in P.cornutum,and providing a reference for molecular breeding of salt-tolerance in P.cornutum.【Methods】The leaf fresh weight ratio of P.cornutum seedlings at the 6-leaf-1-heart stage was measured after 8 days of treatment with 0,50,100,150,and 200 mmol/L NaCl.The salt concentration corresponding to approximately 50%of the fresh weight ratio was chosen as the treatment concentration for transcriptome analysis,with sampling conducted at 0,3,and 12 h for transcriptome sequencing.【Results】The salt threshold of P.cornutum was determined to be 100 mmol/L by measuring the leaf fresh weight ratio;compared with the 0 h,transcriptomic data at 3 and 12 h identified 10006 and 14117 differentially expressed genes,respectively;a total of four upregulated genes were screened among the transcription factors.In the plant hormone signal transduction process,brassinosteroid(BR)had the highest number of upregulated genes;three key upregulated enzymes were identified in the phenylpropanoid metabolic pathway,and four key upregulated enzymes were identified in the flavonoid biosynthesis pathway.【Conclusion】The upregulated genes involved in transcription factors,the plant hormone signal transduction process,the phenylpropanoid metabolic pathway,and the flavonoid biosynthesis pathway may play critical roles in the response of P.cornutum to salt stress.
作者
商凯凡
赵昀暐
廉勇
王萍
SHANG Kaifan;ZHAO Yunwei;LIAN Yong;WANG Ping(College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Hohhot 010018,China;Institute of Agricultural and Livestock Products Processing,Inner Mongolia Academy of Agricultural&Animal Husbandry Sciences,Hohhot 010031,China)
出处
《北方农业学报》
2025年第4期49-66,共18页
Journal of Northern Agriculture
基金
国家自然科学基金项目(31860553,31260475)。
关键词
沙芥
转录组
盐胁迫
耐盐基因
Pugionium cornutum
Transcriptome
Salt stress
Salt-tolerance gene
