摘要
近年来胸膜肺炎放线杆菌(APP)引起的猪传染性胸膜肺炎在我国规模化猪场流行率显著升高,对新流行的菌株进行分离鉴定和生物学特性检测有重要的临床意义。本研究从湖北地区某些疑似暴发猪传染性胸膜肺炎的猪场肺脏病料中分离了4株疑似APP菌株(命名为XB2T-56、JMTLP-443、YD-THB-755、SYZJ-291),对其进行了PCR扩增、多重PCR血清分型等鉴定,检测了分离株的生长能力、溶血活性、生物被膜形成能力等生物学特性,通过药敏试验分析了菌株的耐药情况,并对其中1株菌(XB2T-56)进行了小鼠和仔猪毒力评价。PCR鉴定结果显示,4株临床分离株均为APP血清15型菌株,这4株菌生长速度慢于1型标准菌株,溶血活性较弱,但能形成较强的生物被膜。这些菌株均对头孢氨苄、泰乐菌素、磺胺二甲嘧啶、多西环素敏感性较低,而对头孢噻呋、恩诺沙星敏感。小鼠和仔猪感染试验结果显示,分离株XB2T-56具有较强的毒力,人工鼻内感染引起仔猪急性死亡,肺部呈典型的胸膜肺炎症状,并且能够从病变处分离到感染的血清15型菌株。结果表明,APP血清15型菌株具有较强的毒力,为APP的临床流行特征提供了依据。
In recent years,the prevalence of porcine infectious pleuropneumonia caused by Actinobacillus pleuropneumoniae(APP)has significantly increased in large-scale pig farms in China.The isolation,identification,and biological characteristic testing of newly prevalent strains have important clinical significance.This study isolated four suspected APP strains(named XB2T-56,JMTLP-443,YD-THB-755,SYZJ-291)from lung samples of pig farms suspected of outbreaks of porcine infectious pleuropneumonia in Hubei Province.They were identified by PCR amplification,and multiplex PCR serological typing,the biological properties were tested by detecting the growth ability,hemolytic activity and biofilm forming ability.The drug resistance of the strains was analyzed by drug sensitivity testing,and the virulence of one strain(XB2T-56)in mice and piglets was evaluated.The PCR identification result showed that all four clinical isolates were APP serotype 15strains.These four strains grew slower than serotype 1standard strains,had weaker hemolytic activity,but could form stronger biofilms.These strains are all less sensitive to cephalosporin,tylosin,sulfamethoxazole,and doxycycline,but sensitive to cefotaxime and enrofloxacin.The mice and piglet infection experiments showed that the isolated strain XB2T-56has strong virulence.Artificial intranasal infection causes acute death in piglets,with typical symptoms of pleuropneumonia in the lungs,and serotype 15strains can be isolated from the lesion site.This study indicates that the APP serotype 15strain has strong virulence,providing new date for the clinical epidemiological characteristics of APP.
作者
朋璐
张娇
韩未尧
张秋红
罗侦
洪勃
刘志昌
周锐
宋云峰
黎璐
PENG Lu;ZHANG Jiao;HAN Weiyao;ZHANG Qiuhong;LUO Zhen;HONG Bo;LIU Zhichang;ZHOU Rui;SONG Yunfeng;LI Lu(The Cooperative Innovation Center for Sustainable Pig Production Co-sponsored by Province and Ministry/National Key Laboratory of Agricultural Microbiology,College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;Shanghai Key Laboratory of Veterinary Biotechnology,Shanghai 200240,China)
出处
《中国兽医学报》
北大核心
2025年第10期2156-2162,2230,共8页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(32473032)
上海市兽医生物技术重点实验室开放基金资助项目(BD1500010)。
关键词
胸膜肺炎放线杆菌
临床分离株
15型
生物学特性
毒力
Actinobacillus pleuropneumoniae
clinical isolates
serotype 15
biological characteristics
virulence in piglets
