摘要
为了探究转录调节因子LIM同源盒蛋白8基因(LIM homebox protein 8, LHX8)内含子5多态性与松辽黑猪繁殖性状的关联性,试验选取120头经产松辽黑猪母猪为研究对象,采用Sanger直接测序法检测LHX8基因第5内含子的单核苷酸多态性(SNP)位点,使用SPSS 22.0分析LHX8基因内含子5的SNP与松辽黑猪繁殖性状的关联性。结果表明,松辽黑猪LHX8基因内含子5存在T/C突变;共检测到TT、TC和CC共3种基因型,T、C两种等位基因,优势等位基因型为CC,优势等位基因为C。χ^(2)适合性检验表明,该SNP在松辽黑猪群体中处于Hardy-Weinberg平衡状态,为中度多态(0.25<PIC<0.5)。关联分析结果表明,CC基因型个体断奶仔猪数显著多于TC基因型(P<0.05)。TC基因型个体初生重极显著高于TT基因型与CC基因型(P<0.01),CC基因型显著高于TT基因型(P<0.05)。说明松辽黑猪LHX8基因第5内含子T30C是潜在的与繁殖性状相关的遗传位点。但该突变位点能否作为松辽黑猪繁殖性状的遗传标记,需要进行更大规模的群体验证。
This study aimed to investigate the association between the polymorphism of intron 5 of LIM homebox pro⁃tein 8(LHX8)gene and reproductive traits in Songliao black pigs.A total of 120 multiparous Songliao Black sows were genotyped for single nucleotide polymorphisms(SNPs)in intron 5 ofLHX8 using Sanger direct sequencing,and the correlations between SNPs and reproductive traits were analyzed via SPSS 22.0.A T/C mutation was identified in intron 5 ofLHX8,with three genotypes(TT,TC,and CC)and two alleles(T and C)detected.The dominant geno⁃type and allele were CC and C,respectively.Chi-square goodness-of-fit test showed that the SNP was in Hardy-Weinberg equilibrium and exhibited moderate polymorphism(0.25<PIC<0.5).Association analysis revealed that sows with the CC genotype had significantly more weaned piglets than those with the TC genotype(P<0.05).Birth weight was significantly higher in the TC genotype than in both TT and CC genotypes(P<0.01),with CC significantly higher than TT(P<0.05).These results indicate that the T30C mutation in intron 5 ofLHX8 is a potential genetic lo⁃cus associated with reproductive traits in Songliao black pigs.However,validation in larger populations is required to confirm its utility as a genetic marker for reproductive traits.
作者
张琪
张方玮
刘庆雨
张树敏
李娜
于永生
ZHANG Qi;ZHANG Fangwei;LIU Qingyu;ZHANG Shumin;LI Na;YU Yongsheng(Jilin Academy of Agricultural Sciences(Northeast Agricultural Research Center of China),Gongzhuling 136100;College of Animal Science and Technology,Jilin Agricultural University,Changchun 130118,China)
出处
《东北农业科学》
2025年第4期81-85,90,共6页
Journal of Northeast Agricultural Sciences
基金
吉林省科技厅重点研发项目(20220202039NC)
