摘要
肺癌是全球范围内发病率和死亡率最高的恶性肿瘤之一,其精准诊疗与药物开发亟需能够高度模拟人体内真实病理生理特征的体外模型。类器官芯片作为一种新兴技术平台,通过微流控工程、生物材料等多种工程技术手段整合类器官培养,能够精确控制细胞微环境,从而在体外高度模拟人体器官的三维结构与生理功能。该类系统在肿瘤研究、发育生物学和疾病建模等领域展现出显著优势,不仅可保留患者样本异质性和病理特征,还支持多种细胞共培养,重构肿瘤微环境(tumor microenvironment, TME)。然而,目前该技术在肺癌研究中的标准化构建方法与整合分析策略尚待完善。本综述系统阐述了类器官芯片的关键技术原理、在肺癌模型构建、药物筛选及免疫治疗研究中的最新进展,旨在为推动其在肺癌精准医疗与转化研究中的深入应用提供理论依据与技术展望。
Lung cancer remains one of the most prevalent and lethal malignancies worldwide.The advancement of its precise diagnosis and therapeutic development urgently requires in vitro models that can highly recapitulate the pathophysiological characteristics of human tissues.Organ-on-a-chip has emerged as a novel technological platform that integrates microfluidic engineering,biomaterials,and other engineering strategies with organoid culture.This platform enables precise control over the cellular microenvironment,thereby closely mimicking the three-dimensional structure and physiological functions of human organs in vitro.Organ-on-a-chip systems demonstrate significant advantages in cancer research,developmental biology,and disease modeling,as they not only preserve the heterogeneity and pathological features of patient samples but also support co-culture of various cell types to reconstruct the tumor microenvironment(TME).However,standardized construction methods and integrated analytical strategies for this technology in lung cancer research remain to be further refined.This review systematically elaborates on the key technical principles of organ-on-a-chip and its recent advances in lung cancer modeling,drug screening,and immunotherapy research.It aims to provide a theoretical foundation and technical perspective for promoting the deeper application of organ-on-a-chip in precision medicine and translational research for lung cancer.
作者
贠武阳
张效云
肖漓
Wuyang YUN;Xiaoyun ZHANG;Li XIAO(College of Pulmonary and Critical Care Medicine,Beijing Key Laboratory of Organ Transplantation and Immunology Regulatory,The 8^(th)Medical Centre of Chinese PLA General Hospital,Beijing 100091,China;Hebei North University,Zhangjiakou 075000,China)
出处
《中国肺癌杂志》
北大核心
2025年第9期689-699,共11页
Chinese Journal of Lung Cancer
关键词
肺肿瘤
类器官
类器官芯片
免疫治疗
抗肿瘤药物筛选
Lung neoplasms
Organoid
Organoid-on-a-chip
Immunotherapy
Antitumor drug screening assays
