摘要
为了获得绿孢链霉菌降解光氧化褐煤过程的热力学信息以及降解效果,采用等温微量热法研究绿孢链霉菌对光氧化内蒙古胜利褐煤的降解过程,并采用溶剂极性由小到大的顺序逐级对降解产物进行萃取,萃取物通过GC-MS进行鉴定分析。结果表明:内蒙古胜利褐煤的降解过程可分为4个阶段:迟缓期0~2.8 d(AB段)、指数生长期2.8 d~4.6 d(BC段)、稳定期4.6 d~8.0 d(CD段)和衰亡期8.0 d~12.9 d(DE段),降解过程中释放的热量为51.46 kJ/g,降解时间为12.9 d,绿孢链霉菌的生长速率常数k=9.7308×10^(-5 )min^(-1);降解液溶剂萃取物的GC-MS检测结果表明,降解液中含有化合物65种,极性最小的正己烷萃取物中主要为极性较小的烷烃,极性较大的甲苯和乙酸乙酯萃取物中主要为酯和杂环化合物,可见溶剂逐级萃取可对降解液进行初步分离。
In order to obtain the thermodynamic information and degradation efficiency on the degradation of photo-oxidized lignite by streptomyces viridosporous,isothermal microcalorimetry was employed to study the degradation process of photo-oxidized Shengli lignite from Inner Mongolia by streptomyces viridosporous.The degradation products were sequentially extracted using solvents with increasing polarity,and the extracts were identified and analyzed via GC-MS.The results showed that the degradation process of Inner Mongolia Shengli lignite could be divided into four stages:the lag phase(0-2.8 days,AB segment),the exponential growth phase(2.8-4.6 days,BC segment),the stationary phase(4.6-8.0 days,CD segment),and the decline phase(8.0-12.9 days,DE segment).The heat released during the degradation process was 51.46 kJ/g,with a degradation duration of 12.9 days.The growth rate constant(k)of streptomyces viridosporous was determined to be 9.7308×10^(-5 )min^(-1).GC-MS analysis of the solvent extracts from the degradation solution revealed the presence of 65 compounds.The hexane extract with the lowest polarity primarily contained less polar alkanes,while the toluene and ethyl acetate extracts with higher polarity mainly contained esters and heterocyclic compounds,demonstrating that sequential solvent extraction could achieve preliminary separation of the degradation solution.
作者
李建涛
杨宝婷
木欣凯
刘向荣
Li Jiantao;Yang Baoting;Mu Xinkai;Liu Xiangrong(College of Chemical Engineering and Modern Materials/Shaanxi Engineering Research Center for Mineral Resources Clean&Efficient Conversion and New Materials,Shangluo University,Shangluo Shaanxi 726000,China;College of Chemistry and Chemical Engineering,Xi′an University of Science and Technology,Xi′an Shaanxi 710054,China;Key Laboratory of Coal Resources Exploration and Comprehensive Utilization of MLR,Xi′an Shaanxi 710021,China)
出处
《煤化工》
2025年第5期40-44,共5页
Coal Chemical Industry
基金
国家自然科学基金项目(U19031332,21373158)
陕西省国际合作重点项目(2023-GHZD-16)
陕西省大学生创新创业训练计划项目(S202311396045)。
关键词
等温微量热法
光氧化褐煤
绿孢链霉菌
降解
产物
isothermal microcalorimetry
photo-oxidized lignite
streptomyces viridosporous
degradation
product
