摘要
建兰花叶病毒(Cymbidium mosaic virus,CymMV)和齿兰环斑病毒(Odontoglossum ringspot virus,ORSV)是蝴蝶兰生产中的常见病毒,对组培苗CymMV和ORSV的携带情况进行监测是生产蝴蝶兰优质健康种苗的前提。本研究以20个蝴蝶兰品种为研究对象,从组培生产线上选取继代增殖周期间隔10个月的2个批次组培苗样品,同时采用RT-PCR和RT-qPCR进行CymMV和ORSV检测。RT-PCR检测结果显示,第1批次和第2批次样品的CymMV阳性率分别为25%和20%,ORSV阳性率均为15%。RT-qPCR检测结果显示,第1批次和第2批次样品的CymMV阳性率分别为100%和91.67%,ORSV阳性率分别为50%和25%。与第1批次相比,第2批次组培苗分别有16个和9个品种的CymMV和ORSV含量减少,但V3的ORSV含量增加明显,由阴性增加至6.85×10^(5)~8.50×10^(5) copies/μL。研究结果表明,CymMV和ORSV广泛存在于蝴蝶兰组培苗中,且病毒含量随批次存在差异,检测为阴性的品种在继代增殖过程中亦存在病毒量增加的风险。因此,在蝴蝶兰组培苗生产过程中应定期进行CymMV和ORSV监测,以保证蝴蝶兰产业的优质种源。
Cymbidium mosaic virus(CymMV)and Odontoglossum ringspot virus(ORSV)are common viruses in Phalaenopsis production,and monitoring CymMV and ORSV of in vitro seedlings is the foundation of high-quality and healthy seedling production in Phalaenopsis.In this study,20 Phalaenopsis cultivars were used as the research objects.Two batches of in vitro seedlings selected from the production line with 10-month intervals between the subculture proliferation cycles,were investigated for CymMV and ORSV through RT-PCR(reverse transcription polymerase chain reaction)and RT-qPCR(real time quantitative polymerase chain reaction)at the same time.RT-PCR results showed that the positive rate of CymMV in the first and second batch was 25%and 20%,respectively,and the positive rate of ORSV was both 15%in the two batches.RT-qPCR results showed that the positive rate of CymMV in the first and second batch was 100%and 91.67%,respectively,and the positive rate of ORSV in the two batches was 50%and 25%,respectively.Comparing with the first batch,CymMV content of 16 cultivars and ORSV content of 9 cultivars decreased in the second batch,but ORSV content of Phalaenopsis Sogo Yukidian V3 increased significantly,which increased from negative to 6.85×10^(5)~8.50×10^(5) copies/µL.The results indicated that CymMV and ORSV widely existed in in vitro seedlings of Phalaenopsis,and the content varied with different batches.The cultivars detected as the negative also faced the risk of virus content increase.Thus,CymMV and ORSV should be investigated regularly in the production of in vitro Phalaenopsis seedlings to ensure the high-quality seedling source for Phalaenopsis industry.
作者
冯可婧
许丽
杨春梅
阮继伟
吴丽芳
瞿素萍
余蓉培
FENG Kejing;XU Li;YANG Chunmei;RUAN Jiwei;WU Lifang;QU Suping;YU Rongpei(College of Agriculture,Yunnan University,Kunming,Yunnan 650504,China;Institute of Flower Research,Yunnan Academy of Agricultural Sciences/Key Laboratory of Flower Breeding of Yunnan Province/National Engineering Research Center for Ornamental Horticulture,Kunming,Yunnan 650205,China;Yunxing Biotechnology Co.,Ltd.,Yuxi,Yunnan 653100,China)
出处
《热带作物学报》
北大核心
2025年第11期2722-2732,共11页
Chinese Journal of Tropical Crops
基金
云南省种子种业联合实验室项目(No.202205AR070001-7)
云南省应用基础研究计划项目(No.202501AT070088)
云南省乡村振兴科技专项(No.202304BI090016)。
关键词
蝴蝶兰
建兰花叶病毒
齿兰环斑病毒
组培苗
Phalaenopsis
Cymbidium mosaic virus
Odontoglossum ringspot virus
in vitro seedlings
