摘要
以农业农村部景洪澳洲坚果种质资源圃内200份国内外澳洲坚果种质的10个花序性状,使用QGAStation软件组合筛选不同遗传距离、聚类方法、取样策略和取样比例,进行核心种质构建探究。澳洲坚果基于花序表型的核心种质最佳构建方法为“马氏距离+多次聚类偏离度取样法+重心法聚类+15%取样比例”,构建了包含30份资源的核心种质。核心种质和原始种质的均值差异百分率、方差差异百分率、极差符合率和变异系数变化率分别为0.00%、90%、99.31%和150.08%。核心种质与原始种质各性状均值T检验无显著差异,方差均大于原始种质,其中9个性状的方差达到显著水平;遗传多样性指数较原始种质有所减小,但符合率均大于80%。主成分分析显示,原始种质和核心种质前3个主成分累计方差贡献率分别为74.118%和84.350%,核心种质的主成分得分图几何结构与原始种质一致。相关性分析显示核心种质各性状相关关系与原始种质较为一致。核心种质由8份澳大利亚种质、10份夏威夷种质、9份中国云南种质和3份特殊种质构成,存在一定地域性。核心种质的聚类和主成分分析显示,国内和国外种质可依据花序表型进行一定程度的区分。本研究基于花序表型构建的澳洲坚果核心种质能较好代表原始种质,可为澳洲坚果种质资源研究提供依据。
10 inflorescence traits of 200 domestic and international Macadamia germplasms in the Jinghong Macadamia Germplasm Repository of the Ministry of Agriculture and Rural Affairs were evaluated using QGA Station combined with different genetic distance metrics,clustering methods,sampling strategies,and sampling proportions to conduct a core macadamia collection construction.The optimal methodology for core collection development was identified as"Mahalanobis distance+multi-clustering deviation sampling+centroid clustering method+15%sampling proportion",resulting in a core collection comprising 30 accessions.The mean difference percentage,variance difference percentage.range coincidence rate and variation coefficient change of core collection and original collection was O.00%,90.00%,99.31%and 150.08%,respectively.No significant differences in trait means were detected between the core and original collections(T-test).The variances of all traits in the core collection exceeded those of the original collection,with 9traits showing statistically significant differences.While genetic diversity indices slightly decreased,the conformity rate for all traits remained above 80%.Principal component analysis(PCA)demonstrated that the cumulative variance contribution rate of the first three principal components was 74.118%and 84.350%for the original and core collections,respectively,with congruent geometric structures in their PCA scatterplots.Correlation analysis further confirmed that trait relationships in the core collection closely mirrored those of the original collection.The core collection comprised 8 Australian accessions,10 Hawaiian accessions,9 China Yunnan accessions,and 3 accessions of special types,exhibiting a degree of geographic representativeness.Analyses using both cluster analysis and PCA on the core collection revealed that domestic and foreign accessions were distinguishable to a certain extent based on the inflorescence phenotypes.The Macadamia core collection developed in this study,based on inflorescence phenotypic traits,effectively represents the genetic diversity of the original collection and would provide a robust foundation for future research on Macadamia genetic resources.
作者
李扬
宫丽丹
马静
龙青姨
李志强
耿建建
吴超
李婷玉
罗勇
黄妮娅
陶亮
LI Yang;GONG Lidan;MA Jing;LONG Qingyi;LI Zhiqiang;GENG Jianjian;WU Chao;LI Tingyu;LUO Yong;HUANG Niya;TAO Liang(Yunnan Institute of Tropical Crops,Jinghong,Yunnan 666100,China;Macadamia Agricultural Engineering Research Center of Yunnan,Jinghong,Yunnan 666100,China;Jinghong City Forestry and Grassland Bureau,Jinghong,Yunnan 666100,China)
出处
《热带作物学报》
北大核心
2025年第11期2592-2602,共11页
Chinese Journal of Tropical Crops
基金
云南省第十二批技术创新人才培养对象项目(No.202305AD160033)
云南省热带作物科学研究所热带作物科技创新专项资金项目(No.RF2025)。
关键词
澳洲坚果
种质资源
花序性状
核心种质
Macadamia
germplasm resources
inflorescence traits
core collection
