摘要
目的:探讨与分析结直肠癌(Colorectal Cancer,CRC)组织中错配修复(Mismatch repair,MMR)蛋白、程序性死亡配体1(Programmed death ligand 1,PD-L1)表达与临床病理特征的相关性。方法:研究时间为2021年7月至2024年12月,选择在本院诊治的结直肠癌患者200例作为研究对象。取所有患者病灶组织标本(病灶组)与癌旁组织标本(距肿瘤边缘≥5 cm,癌旁组),采用免疫组织化学(Immunohistochemistry,IHC)方法检测错配修复(Mismatch repair,MMR)(MLH1、MSH2、MSH6和PMS2)蛋白与PD-L1蛋白表达情况。调查患者的临床病理特征并进行相关性分析。结果:病灶组的dMMR与pMMR蛋白表达阳性率为39.00%、70.00%,癌旁组为11.00%、89.00%,病灶组与癌旁组相比有明显差异(P<0.05)。病灶组的PD-L1蛋白表达阳性率为25.00%,癌旁组为80.00%,病灶组与癌旁组相比显著降低(P<0.05)。不同组织学分化、临床分期、淋巴结转移患者的MMR与PD-L1蛋白表达阳性率对比有明显差异(P<0.05),不同性别、年龄、发病部位患者的MMR与PD-L1蛋白表达阳性率无明显差异(P>0.05)。Spearman分析结直肠癌组织MMR与PD-L1蛋白表达与组织学分化、临床分期、淋巴结转移等临床病理特征存在相关性(P<0.05)。结论:结直肠癌组织中多呈现MMR与PD-L1蛋白低表达状况,结直肠癌组织MMR与PD-L1蛋白表达与组织学分化、临床分期、淋巴结转移等临床病理特征存在相关性。
Objective:To explore and analyze the correlation between the expression of mismatch repair(MMR)protein and programmed death ligand 1(PD-L1)in colorectal cancer(CRC)tissue and clinical pathological characteristics.Methods:The study period were from July 2021 to December 2024,200 cases of colorectal cancer patients treated in our hospital were selected as the research subjects.Collected all patient lesion tissue samples(lesion group)and adjacent tissue samples(located≥5 cm from the tumor edge,adjacent group),and used the immunohistochemistry(IHC)method to detect the expression of Mismatch repair(MMR)(MLH1,MSH2,MSH6,and PMS2)protein and PD-L1 protein.Investigated the clinical and pathological characteristics of patients and conducted correlation analysis.Results:The positive rates of dMMR and pMMR protein expression in the lesion group were 39.00%and 70.00%,respectively,while those in the adjacent cancer group were 11.00%and 89.00%,respectively.There were significant difference compared between the lesion group and the adjacent cancer group(P<0.05).The positive rate of PD-L1 protein expression in the lesion group were 25.00%,while that in the adjacent cancer group were 80.00%.The expression of PD-L1 protein in the lesion group were significantly lower than that in the adjacent cancer group(P<0.05).There were significant differences in the positive rates of MMR and PD-L1 protein expression among patients with different histological differentiation,clinical stages,and lymph node metastasis(P<0.05),while there were no significant differences in the positive rates of MMR and PD-L1 protein expression among patients of different genders,ages,and disease sites(P>0.05).Spearman analysis showed there were correlation between the expression of MMR and PD-L1 proteins in colorectal cancer tissues and clinical pathological features such as histological differentiation,clinical staging,and lymph node metastasis(P<0.05).Conclusion:Low expression of MMR and PD-L1 proteins are commonly observed in colorectal cancer tissues.The expression of MMR and PD-L1 proteins in colorectal cancer tissues are correlated with clinical pathological features such as histological differentiation,clinical staging,and lymph node metastasis.
作者
孟宪杰
肖莉
张海俊
胡建明
魏莉
MENG Xian-jie;XIAO Li;ZHANG Hai-jun;HU Jian-ming;WEI Li(Department of Pathology,Shihezi People's Hospital,Shihezi,Xinjiang,832000,China;Department of Ultrasound Diagnosis,Shihezi People's Hospital,Shihezi,Xinjiang,832000,China;Department of Pathology,The First Affiliated Hospital of Shihezi UniversityMedical College,Shihezi,Xinjiang,832008,China)
出处
《现代生物医学进展》
2025年第19期3056-3061,3113,共7页
Progress in Modern Biomedicine
基金
2023年度兵团指导性科技计划项目(2023ZD027)。
关键词
结直肠癌
错配修复
组织学分化
程序性死亡配体1
淋巴结转移
相关性
Colorectal cancer
Mismatch repair
Histological differentiation
Programmed death ligand 1
Lymph node metastasis
Correlation
