摘要
目的探究聚羟基乙酸(polyglycolic acid,PGA)与羟丙基甲基纤维素(hydroxypropyl methylcellulose,HPMC)复合材料用于犬胃管状切除术后缝合的有效性和安全性。方法选取36只疫检合格的比格犬,按雌雄1∶1随机分为实验组和对照组。实验组在胃管状切除术后采用PGA+HPMC复合材料进行切口缝合加固,对照组采用NV-ET-M60E-2缝合线。术后监测比格犬生存情况、进食情况及体温变化,并在术前、术后3 d及解剖前采集静脉血进行血常规检测。术后2、4、8周解剖比格犬,每个时间点解剖6只比格犬,取心、肝、脾、肺、肾进行组织学检查、组织病理切片病理诊断及安全性评估。结果对照组和实验组的血常规检测结果无统计学差异(P>0.05)。解剖前,对照组嗜碱性粒细胞百分数显著高于实验组(P=0.015)。术后2周时,对照组比格犬肝细胞水肿严重程度高于实验组(P=0.036),但其他各项心、肝、脾、肺、肾组织学检测结果在两组之间无统计学差异(P>0.05)。术后2、4、8周的硬组织病理切片结果显示,两组材料在第8周均无残留,炎症与坏死评分和纤维化/脂肪浸润总分均无统计学差异(P>0.05)。炎症评分和纤维化/脂肪浸润总分在术后8周显著低于术后2周(P<0.05)。实验过程中,两组比格犬均无濒死现象,术后3 d内无拒绝禁食和发热现象,解剖时比格犬外观正常,毛发有光泽,无异常分泌物。结论PGA+HPMC复合材料可作为一种可有效降解且无毒副作用的缝合固定材料运用于胃管状切除后切口缝合。
Objective To explore the effectiveness and safety of polyglycolic acid(PGA)and hydroxypropyl methylcellulose(HPMC)composite materials for suturing after canine sleeve gastrectomy.Methods A total of 36 healthy Beagle dogs were randomly divided into experimental group and control group in a 1∶1 male-to-female ratio.The experimental group underwent gastric sleeve resection with PGA+HPMC composite material for suture reinforcement,while the control group used NV-ET-M60E-2 suture material.Postoperative monitoring included survival status,food intake,and body temperature changes.Venous blood samples were collected before surgery,3 days after surgery,and before euthanasia for routine blood tests.At 2,4,and 8 weeks postoperatively,six dogs were euthanized at each time point for histological examination,hard tissue pathological evaluation,and safety assessment of the heart,liver,spleen,lungs,and kidneys.Results There were no significant differences in blood routine test results between the control group and the experimental group(P>0.05).Before dissection,the percentage of basophils was significantly higher in the control group than in the experimental group(P=0.015).At 2 weeks after surgery,the severity of liver cell edema in the control group was higher than in the experimental group(P=0.036).However,the two groups did not significantly differ in the histological examination results of the heart,liver,spleen,lungs,or kidneys at other time points(P>0.05).Hard tissue pathological slice results at 2,4,and 8 weeks after surgery showed no material residue in either group by the 8th week,and there were no significant differences in inflammation and necrosis scores or fibrosis/fat infiltration totals between the two groups(P>0.05).Inflammation scores and fibrosis/fat infiltration totals at 8 weeks post-operation were significantly lower than those at 2 weeks post-operation(P<0.05).During the experiment,no near-death phenomena occurred in either group,and no fever or refusal to eat was observed within 3 days after surgery.At dissection,the Beagle dogs appeared normal with glossy fur and no abnormal secretions.Conclusion PGA+HPMC composite materials can be effectively degraded and used as non-toxic suture fixation materials for wound suturing after sleeve gastrectomy,providing a theoretical basis for their clinical application.
作者
张风华
吴雪烁
王宝枝
夏宇晓
松谷哲行
冯巨滨
ZHANG Fenghua;WU Xueshuo;WANG Baozhi;XIA Yuxiao;Matsutani Noriyuki;FENG Jubin(Department of General Surgery,The Second Affiliated Hospital of Guangzhou Medical University,Panyu Campus,Guangzhou 510000,China;Department of Thoracic Surgery,Gokokuji Hospital,Teikyo University School of Medicine,Tokyo 213-8507,Japan;Department of Gastroenterology,The Second Affiliated Hospital of Guangzhou Medical University,Panyu Campus,Guangzhou 510000,China)
出处
《西安交通大学学报(医学版)》
北大核心
2025年第6期953-959,共7页
Journal of Xi’an Jiaotong University(Medical Sciences)
基金
广东省医学科研基金资助(No.A2023475)。
