摘要
为开发具备多种功能的新型伤口敷料,以玉米醇溶蛋白(Zein)、明胶(Gel)、厚朴酚(MAG)为原料,80%乙酸溶液为溶剂,通过静电纺丝技术制备了一种负载MAG的纳米纤维膜。借助场发射扫描电镜、红外光谱仪、接触角测量仪、拉力试验机、紫外-可见分光光度计等仪器分析不同质量比Zein/Gel纺丝溶液对纳米纤维膜的微观形貌及结构、润湿性、水蒸气透过性、力学性能、药物释放性能、生物相容性、溶血性能、抗氧化性和抗菌性能的影响。结果表明:随着纺丝溶液中Gel质量占比减小,溶液黏度下降,Zein/Gel/MAG纳米纤维直径、水蒸气透过率和弹性模量逐渐减小,而纤维膜水接触角逐渐增大;此外,Zein/Gel/MAG纳米纤维膜中MAG药物释放性能与纤维膜中的Gel质量占比密切相关;Zein/Gel/MAG纳米纤维膜对L929细胞无明显毒性,并且纤维膜溶血率均不超过2%,具有良好的生物相容性;Zein/Gel/MAG纳米纤维膜对1,1-二苯-2-苦基肼自由基均具有一定的清除作用;Zein/Gel/MAG纳米纤维膜对金黄色葡萄球菌和大肠埃希菌均有明显的抑制作用。综上认为,Zein/Gel/MAG纳米纤维膜在伤口敷料方面具有潜在的应用前景。
Objective To overcome the limitations of traditional wound dressings such as poor antibacterial function and the need for frequent replacement,it was necessary to develop innovative multifunctional wound dressings.In this study,a nanofiber membrane loaded with magnolol(MAG)was fabricated using electrospinning technology.The membrane′s morphology,wettability,water vapor transmission rate,mechanical properties,drug release,biocompatibility,hemocompatibility,antioxidant activity and antibacterial efficacy were systematically evaluated to explore its potential application as a wound dressing.Method MAG was dissolved in varying mass ratios of zein/gelatin(Zein/Gel)solutions to prepare precursor solutions for electrospinning.Zein/Gel/MAG composite nanofiber membranes were then fabricated.The nanofibers′microstructure,chemical composition,and wettability were characterized using scanning electron microscopy,Fourier-transform infrared spectroscopy(FT-IR)and contact angle measurements.The effects of different Zein/Gel mass ratios on water vapor transmission rate,mechanical properties,MAG release,cytotoxicity,hemocompatibility,antioxidant activity and antibacterial efficacy were also investigated.Results The fabricated Zein/Gel/MAG nanofiber membranes displayed a smooth surface without obvious bead formation or adhesion,and the nanofiber diameter decreased as the mass proportion of Gel in the spinning solution declined.FT-IR analysis confirmed the presence of Zein,Gel and MAG in the nanofibers.Water contact angle measurements indicated that the nanofiber membranes were hydrophobic,with increasing hydrophobicity accompanying the reduction in the mass proportion of Gel,reaching a maximum value of(116.50±9.24)°.All nanofiber membranes demonstrated water vapor transmission rates above(2527.42±262.94)g/(m^(2)·d),reaching a maximum value of(2805.50±65.17)g/(m^(2)·d).The cumulative release of MAG from nanofiber membranes was strongly correlated with the mass proportion of Gel.Furthermore,the Zein/Gel/MAG nanofiber membranes exhibited no significant cytotoxicity to L929 cells and hemolysis rates of less than 2%.The scavenging rates for 1,1-diphenyl-2-picrylhydrazine free radical were in the range of(32.93±2.22)%to(53.03±8.32)%.The maximum widths of bacteriostatic circle for S.aureus and E.coli were(1.89±0.62)mm and(1.80±0.06)mm,respectively.Conclusion The Zein/Gel/MAG nanofiber membranes were successfully prepared via electrospinning.These membranes exhibited excellent water vapor transmission rate,biocompatibility and hemocompatibility,as well as remarkable antioxidant activity.Specially,the obvious antibacterial efficacies against S.aureus and E.coli were observed.Therefore,the Zein/Gel/MAG nanofiber membrane had significant potential prospect for wound dressings.
作者
吴乐然
吴霓欢
李林耿
钟意
陈鸿鹏
汤南
WU Leran;WU Nihuan;LI Lingeng;ZHONG Yi;CHEN Hongpeng;TANG Nan(School of Pharmacy,Guangdong Medical University,Dongguan,Guangdong 523808,China;School of Biomedical Engineering,Guangdong Medical University,Dongguan,Guangdong 523808,China)
出处
《纺织学报》
北大核心
2025年第10期30-38,共9页
Journal of Textile Research
基金
广东省普通高校重点科研平台和项目(2024ZDZX3040)。
