摘要
节担菌属Wallemia是一类具有典型嗜盐和耐旱特性的丝状真菌,在低水活度环境中具有独特的生态适应性。我国节担菌资源研究相对滞后,目前仅记载2个物种。为系统解析我国节担菌的物种多样性,本研究通过多区域系统采集,从12个省区的不同生态环境中分离获得62株节担菌。基于系统发育分析,这些菌株被鉴定为6个物种,包括Wallemia canadensis、Wallemia hederae、Wallemia mellicola、Wallemia muriae、Wallemia sebi和Wallemia tropicalis,其中前4个为我国首次报道,扩充了我国节担菌物种多样性。针对4个物种的代表菌株(W.canadensis ZYJ2333、W.mellicola ZYJ2331、W.muriae ZYJ2345和W.sebi ZYJ2323),采用单因素试验结合响应面法优化培养条件。结果表明,节担菌最适生长条件为温度24.9℃、pH 5.2和Na Cl浓度5.8%-6.4%,其中Na Cl浓度对节担菌生长的影响最大。在离液耐受性试验中,节担菌在3.5 mol/L MgSO4或1.4-1.8 mol/L MgCl_(2)溶液中仍能生长,展现出较强的离子胁迫耐受能力。本研究不仅丰富了我国的节担菌资源,还为其高效培养体系的建立和后续功能研究提供了重要的理论基础和数据支撑。
The genus Wallemia comprises a group of filamentous fungi known for their halophilic and xerophilic properties,allowing them to colonize low-water-activity habitats.Research on Wallemia species diversity in China remains limited,with only two species having been previously documented.This study aims at enhancing the understanding of Wallemia species diversity in China through isolating and identifying strains from diverse ecological regions.As a result,62 strains were isolated from 12 provinces and six species,Wallemia canadensis,Wallemia hederae,Wallemia mellicola,Wallemia muriae,Wallemia sebi,and Wallemia tropicalis,were identified based on phylogenetic analysis.Among which the former four species were reported in China for the first time.The culture conditions of the representative strains of the four species(W.canadensis ZYJ2333,W.mellicola ZYJ2331,W.muriae ZYJ2345,and W.sebi ZYJ2323)were optimized using single-factor experiments combined with response surface methodology.The results demonstrated that the growth of the strains was optimal at 24.9℃,pH 5.2,and 5.8%–6.4%NaCl.The NaCl concentration exhibiting the most significant influence on fungal growth.In chaotropic tolerance assays,Wallemia strains maintained growth in solutions containing 3.5 mol/L MgSO4 or 1.4–1.8 mol/L MgCl_(2),highlighting their exceptional tolerance to ionic stress.Wallemia resources in China and efficient cultivation of the species have need of further research.
作者
邢汇佼
杨雅暄
李佳妮
张姝
张永杰
XING Huijiao;YANG Yaxuan;LI Jiani;ZHANG Shu;ZHANG Yongjie(School of Life Sciences,Shanxi University,Taiyuan 030006,Shanxi,China)
出处
《菌物学报》
北大核心
2025年第10期19-30,共12页
Mycosystema
基金
农业农村部农业微生物资源收集与保藏重点实验室开放基金项目(KLMRCP2023-01)
山西省基础研究计划(202203021221036)
山西省“三晋英才”计划。
关键词
节担菌
资源收集
生物学特性
离液耐受性
Wallemia
resource collection
biological characteristics
chaotropic tolerance
