摘要
In this paper,based on the association of methylenetetrahydrofolate reductase(MTHFR)with type 2 diabetes(T2D),we evaluated the association of polymorphism with morbidly obesity on risk type 2 diabetes.A case-control study of 74 health morbidly obese and 76 healthy non-obese was conducted in Iraq.MTHFR(C677T,A1298C and G1793A)genotyping was performed by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).The study revealed a significant association between cytidine/thymine(CT)genotype of C677T and morbid obesity(odds ratio(OR):1.26,95%confidence interval(CI):0.91-1.60,probability(P)=0.0003),but did not show any significant association in thymine/thymine(TT)genotype(OR:0.258,95%CI:0.88-1.60,P=0.082).On the other hand the second single nucleotide polymorphism(SNP)A1298C showed highly significant association in adenine/adenine(AA)genotype(OR:1.39,95%CI:0.91-1.58,P=0.0001)and significant association between adenine/cytidine(AC)and cytidine/cytidine(CC)genotypes(OR:0.702,95%CI:0.88-1.61,P=0.0377),(OR:0.844,95%CI:0.91-1.59,P=0.0273),respectively,but showed no significant relation in three types of normal homozygous,heterozygous and rare homozygous in MTHFR-G1793A.The results suggested that A1298C substitution might pose a direct effect on being type 2 diabetes in morbidly obese patients,and C677T had moderate effect,while G1793A had no effect.However,further case-control studies are required to provide a more robust conclusion.
