摘要
目的探讨女贞子外囊泡(FELNs)对秀丽隐杆线虫(C.elegans)的寿命调控作用,并初步解析其分子机制。方法采用超速离心法提取FELNs,并通过透射电镜和纳米颗粒追踪分析进行鉴定。以野生型N2线虫为模型,设置不同浓度FELNs干预组,通过寿命实验、氧化应激实验和热应激实验评估其生物学效应。利用转录组测序分析其调控的关键基因与信号通路。结果成功分离的FELNs呈典型杯状结构,平均粒径为148.3nm,线虫可有效摄取。与对照组相比,100μg/mL FELNs处理显著延长线虫最长寿命,并显著提高其在氧化及热应激条件下的存活率。转录组分析显示,FELNs干预后共有291个差异表达基因,主要富集于神经信号传导、先天免疫及自噬等通路。其中,eat-4(谷氨酸转运调控)、col-10(胶原合成)、cpr-2(应激响应)等基因显著上调。结论FELNs可通过多靶点调控神经传递、免疫应答及自噬通路,从而显著延长线虫寿命并增强其抗逆能力。该研究为植物源性纳米囊泡在抗衰老领域的应用提供了新的实验依据。
Objective To investigate the effect of Fructus Ligustri Lucidi exosome-like nanovesicles(FELNs)on the lifespan regulation of Caenorhabditis elegans(C.elegans)and preliminarily elucidate its molecular mechanism.Methods FELNs were extracted by ultracentrifugation and characterized by transmission electron microscopy(TEM)and nanoparticle tracking analysis(NTA).Wild-type N2 C.elegans were used as the model organism,and different concentrations of FELNs were administered to intervention groups.The biological effects were evaluated through lifespan assays,oxidative stress tests,and heat stress assays.Key genes and signaling pathways regulated by FELNs were analyzed using transcriptome sequencing.Results The successfully isolated FELNs exhibited a typical cupshaped structure with an average particle size of 148.3 nm and were effectively taken up by the nematodes.Compared with the control group,treatment with 100μg/mL FELNs significantly extended the maximum lifespan of the nematodes and markedly improved their survival rates under oxidative and heat stress conditions.Transcriptomic analysis revealed 291 differentially expressed genes(DEGs)after FELNs intervention,primarily enriched in pathways such as neural signaling,innate immunity,and autophagy.Among these,genes including eat-4(glutamate transport regulation),col-10(collagen synthesis),and cpr-2(stress response)were significantly upregulated.Conclusion FELNs can significantly extend the lifespan of nematodes and enhance their stress resistance by multi-target regulation of neurotransmission,immune response,and autophagy pathways.This study provides new experimental evidence for the application of plant-derived nanovesicles in the field of anti-aging.
作者
王东
李佳礼
范珈鑫
刘畅
朱砂
付颖
刘倩
张丰硕
何才英
吴佩杰
WANG Dong;LI Jiali;FAN Jiaxin;LIU Chang;ZHU Sha;FU Ying;LIU Qian;ZHANG Fengshuo;HE Caiying;WU Peijie(Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)
出处
《时珍国医国药》
北大核心
2025年第21期4066-4074,共9页
JOURNAL OF LI-SHIZHEN TRADITIONAL CHINESE MEDICINE
基金
四川省自然科学基金(2024NSFSC0692)。
关键词
女贞子
外囊泡
秀丽隐杆线虫
寿命
氧化应激
热应激
转录组学
Fructus Ligustri Lucidi
Exosome-like nanovesicles
Caenorhabditis elegans
Lifespan
Oxidative stress
Heat stress
Transcriptomics
