摘要
目的:分析杭州地区2018—2023年人冠状病毒OC43(human coronavirus OC43,HCoV-OC43)的流行病学及分子进化特征,为HCoV-OC43的感染防控提供分子流行病学依据。方法:收集2018年1月—2023年12月杭州市2家哨点医院急性呼吸道感染病例样本11904份,通过实时荧光定量RT-PCR检测HCoV-OC43,采用特异性引物对阳性样本进行PCR扩增,扩增产物使用三代纳米孔技术进行全基因组测序,并进行遗传进化特征分析。结果:共检出HCoV-OC43阳性样本265份,阳性检出率为2.23%(265/11904)。男性与女性检出率差异无统计学意义(χ^(2)=0.042,P>0.05);不同年龄段检出率差异有统计学意义(χ^(2)=60.104,P<0.01),5岁以下儿童和65岁以上老人检出率较高;2018—2019年和2023年的感染高峰在春夏季,2020—2022年的感染高峰在夏秋季。采用高通量测序共获得85株完整的HCoV-OC43全基因组序列,进化分析显示2018—2023年杭州地区以K基因型为主,2019年存在J和K两种基因型共流行,2023年发现了新的基因型,暂将其命名为基因型L,由基因型J和基因型K重组而来。K基因型有22个特异性氨基酸变异位点,且大部分位于刺突蛋白的重要结构域;J基因型有7个特异性氨基酸变异位点,且大部分位于非结构蛋白;新基因型L有10个特异性氨基酸变异位点,且分布在6个蛋白质,突变位点覆盖最广。结论:HCoV-OC43为杭州地区儿童和老人呼吸道疾病的重要病原体,杭州地区存在多个基因型并在2023年发现了新的重组基因型L。
Objective To analyze the epidemiological characteristics and genetic evolution of human coronavirus OC43(HCoV-OC43)in Hangzhou from 2018 to 2023,and provide molecular epidemiological basis for the prevention and control of HCoV-OC43 infection.MethodsA total of 11904 samples of cases with acute respiratory tract infection were collected from two sentinel hospitals in Hangzhou.The collection period was from January 2018 to December 2023.HCoV-OC43 was detected by real-time fluorescent quantitative RT-PCR.The positive samples were amplified by PCR using specific primers,and the amplified products were sequenced using third-generation nanopore technology for the whole genome.The genetic evolution features were analyzed.ResultsA total of 265 HCoV-OC43 positive samples were detected,with a positive detection rate of 2.23%(265/11904).There was no significant difference in detection rate between males and females(χ^(2)=0.042,P>0.05);the detection rate varied significantly among different age groups(χ^(2)=60.104,P<0.01),with higher detection rates observed in children under five years old and individuals over 65 years old.The infection peaks in 2018-2019 and 2023 occurred in spring and summer,while the infection peak in 2020-2022 was in summer and autumn.A total of 85 complete HCoV-OC43 whole genome sequences were obtained through high-throughput sequencing.The evolutionary analysis indicated that the K genotype was predominant in Hangzhou from 2018 to 2023.In 2019,both J and K genotypes co-existed.In 2023,a new genotype,temporarily named genotype L,was discovered,which originated from genotypes J and K.The K genotype had 22 specific amino acid variation sites,most of which were located in the important domain of S protein.The J genotype had seven specific amino acid variation sites,most of which were located in non-structural proteins.The new genotype L had 10 specific amino acid mutation sites,which were distributed across six proteins,and these mutation sites had the most extensive coverage.ConclusionsHCoV-OC43 is an important pathogen causing respiratory diseases in children and the elderly in the Hangzhou area,and there are multiple genotypes in the Hangzhou area,with a new recombinant genotype L discovered in 2023.
作者
周银燕
于新芬
曹飞飞
程实
俞越
李钧
Zhou Yinyan;Yu Xinfen;Cao Feifei;Cheng Shi;Yu Yue;Li Jun(Microbiological Laboratory,Hangzhou Center for Disease Control and Prevention(Hangzhou Health Supervision Institution),Hangzhou 310021,China)
出处
《中华微生物学和免疫学杂志》
北大核心
2025年第10期847-853,共7页
Chinese Journal of Microbiology and Immunology
基金
杭州市医学重点学科(2020-2024)
杭州市医药卫生科技项目(A20200487)
浙江省感染与免疫多组学重点实验室。
关键词
冠状病毒
基因型
进化
变异
Coronavirus
Genotype
Evolution
Mutation
