摘要
目的探讨松花粉对肠道损伤的保护及治疗作用。方法以KM小鼠(n=100)为实验对象,适应性饲养7 d,随机分为5组,分别为空白组、模型组、阳性药物组、松花粉低剂量组和高剂量组。通过给予小鼠葡聚糖硫酸钠(dextran sulfate sodium,DSS)和吲哚美辛建立两种不同因素引起的肠道损伤模型,以灌胃方式给予1 g/kg和2 g/kg的松花粉。采用苏木精-伊红染色(HE)和马松染色(Masson)观察小鼠肠道组织病理形态,采用ELISA试剂盒检测小鼠炎症因子水平。结果与空白组相比,两种不同方式模型组小鼠血清中SOD和GSH-PX含量显著下降(P<0.05),炎症因子TNF-α、IL-6及IL-12显著升高(P<0.05),IL-10及IgA的水平显著下降(P<0.05),肠道长度显著缩短;表明两种造模方式均成功。与DSS模型组相比,松花粉能够减轻小鼠的肠道损伤程度,且高剂量组的治疗效果优于低剂量组,小鼠血清中SOD和GSH-PX的水平显著升高(P<0.05),高剂量组促炎因子IL-6水平显著降低(P<0.01),抗炎因子IgA的水平有升高趋势(P<0.05)。与吲哚美辛模型组相比,松花粉可缓解小鼠肠道损伤状况,高剂量组中SOD和GSH-PX的水平有升高的趋势(P<0.05),促炎因子TNF-α的水平有降低的趋势(P<0.05),抗炎因子IL-10的水平有升高的趋势(P<0.05)。结论松花粉可改善由DSS和吲哚美辛诱导的小鼠肠道损伤状况。由DSS引起的肠道损伤,高剂量组松花粉对其改善效果显著,低剂量组次之。而由吲哚美辛引起的肠道损伤,松花粉对其改善效果不显著,但高剂量仍具有一定作用。本研究为松花粉的综合开发提供了理论依据。
Objective To investigate the protective and therapeutic effects of pinepollen on intestinal damage.Methods A total of 100 KM mice were used as experimental subjects.After 7 days of acclimatization,the mice were randomly divided into 5 groups:the blank group,the model group,the positive drug group,the low-dose pine pollen group,and the high-dose pine pollen group.Two intestinal injury models were induced by different factors were established by administering dextran sulfate sodium(DSS)and indomethacin to the mice,respectively.Pine pollen was given via gavage at doses of 1 g/kg and 2 g/kg.Hematoxylin-eosin(HE)staining and Masson staining were used to observe the pathological morphology of mouse intestinal tissues,and enzyme-linked immunosorbent assay(ELISA)kits were employed to detect the levels of inflammatory factors in the mice.Results Compared with the blank group,the serum levels of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)in the two model groups(established by DSS and indomethacin,respectively)decreased significantly(P<0.05),while the levels of pro-inflammatory factors[tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),and interleukin-12(IL-12)]increased significantly(P<0.05).Additionally,the levels of interleukin-10(IL-10)and immunoglobulin A(IgA)decreased significantly(P<0.05),and the intestinal length was notably shortened,indicating the successful establishment of both intestinal injury models.Compared with the DSS-induced model group,pine pollen alleviated the degree of intestinal injury in mice,with the therapeutic effect of the high-dose pine pollen group was superior to that of the low-dose group.Specifically,the serum levels of SOD and GSH-PX in mice increased significantly(P<0.05);in the high-dose group,the level of the pro-inflammatory factor IL-6 decreased significantly(P<0.01),and the level of the anti-inflammatory factor IgA showed an increasing trend(P<0.05).Compared with the indomethacin-induced model group,pine pollen relieved intestinal injury in mice.In the high-dose pine pollen group,the serum levels of SOD and GSH-PX showed an increasing trend(P<0.05),the level of the pro-inflammatory factor TNF-αexhibited a decreasing trend(P<0.05),and the level of the anti-inflammatory factor IL-10 displayed an increasing trend(P<0.05).Conclusion Pine pollen can improve intestinal injury induced by DSS and indomethacin in mice.For DSS-induced intestinal injury,the high-dose pine pollen group showed a significant improvement effect,followed by the low-dose group.However,for indomethacin-induced intestinal injury,the improvement effect of pine pollen was not significant,though the high-dose pine pollen still exerted a certain protective effect.This study provides a theoretical basis for the comprehensive development of pine pollen.
作者
潘立涛
杨长军
仲米存
尹雷
王莉涵
何梦冉
冯晓敏
王少平
PAN Litao;YANG Changjun;ZHONG Micun;YIN Lei;WANG Lihan;HE Mengran;FENG Xiaomin;WANG Shaoping(School of Pharmacy,Binzhou Medical University,Yantai 264003,Shandong,P.R.China;Yantai New Era Health Industry(Group)Co.,Yantai 264003,Shandong,P.R.China)
出处
《滨州医学院学报》
2025年第5期520-525,共6页
Journal of Binzhou Medical University
基金
山东省科技中小型企业创新能力提升工程项目(2023TSGC0912)。
关键词
松花粉
肠道损伤
葡聚糖硫酸钠
吲哚美辛
pine pollen
intestinal injury
sodium dextran sulfate
indomethacin
