摘要
Microcarriers containing cellulose-derived materials have been successfully applied to enhance the growth of anchorage-dependent cells maintained especially in bioreactors.By replacing microcarriers with nanocarriers containing dextran,we hypothesized that the density of the anchorage-dependent cells would rise dramatically because the decreased particle size and associated enhancement in surface to volume ratios of nanoparticles contained within the nanoemulsion-based nanocarriers would increase the number of dextran molecules for the anchorage-dependent cells to attach to.Our studies utilized self-assembly nanoemulsions(SANE)formed by a modified phase inversion temperature(PIT)process to produce dextran oil and surfactant-containing nanocarriers having mean particle sizes of 26 nm compared to microcarriers which were greater than 6000 nm.Our results demonstrated that dextran-containing nanocarriers allowed up to 10 fold greater cell density,12% more media lactate concentration,83%higher cell lysate protein and 59% greater glucose concentration,used as a measure of polymer levels in the nanocarriers compared to microcarriers.In conclusion,nanocarriers with increased numbers of dextran molecules,developed in these studies may be useful to further increase the production of anchorage-dependent animal cell-derived products or production of mass cell growth for other applications.
