摘要
Surface modification of silver island films(SIFs)was carried out with Biotin-Poly(Ethylene-glycol)-Amine(BEA),which acts as a cross-linker between the silver surface and horse radish peroxidase(HRP)enzyme for optimum plasmon-enhanced enzymatic activity.SIFs-deposited blank glass slides and SIFs-deposited 3-Aminopropyltriethoxysilane(APTES)-coated glass slides were used as our plasmonic surfaces.In this regard,three different extent of loading of SIFs were also prepared(low,medium and high)on APTES-coated glass slides.Streptavidin-linked HRP enzyme was attached to SIFs-deposited blank glass slides and SIFs-deposited APTES-coated glass slides through the well-known biotin-streptavidin interactions.The characterization of these surfaces was done using optical absorption spectroscopy.The loading of SIFs on glass slides was observed to have significant effect on the efficiency of plasmon-enhanced enzymatic activity,where an enhancement of 200%in the enzymatic activity was observed when compared to our previously used strategies for enzyme immobilization in our preceding work[1].In addition,SIFs-deposited on APTES-coated glass slides were found to be re-usable for plasmon-enhanced enzymatic reactions unlike SIFs deposited on to blank glass slides.
基金
supported by Award Number 5-K25EB007565-05 from the National Institute of Biomedical Imaging and Bioengineering.
