摘要
从焦化厂土壤中筛选得到一株多环芳烃降解菌株YS-33通过生理生化和16S rRNA基因序列鉴定该菌为联苯降解菌Dyella ginsengisoli YS-33。采用发酵法得到了该菌的胞外多糖(Dyella ginsengisoli polysaccharides,DGPS)。高效液相色谱(HPLC)分析结果表明,DGPS是由葡萄糖醛酸(GlcAc)和半乳糖(Gal)组成的大分子酸性多糖,GlcAc和Gal的物质的量比为1:6.8。采用高效凝胶渗透色谱法(HPGPC)测定DGPS的分子量为1.71×10^(6),该多糖具有较好的抗氧化性。流变学研究结果显示,DGPS溶液表现出非牛顿流体剪切稀化特性,其表观黏度存在浓度和温度依赖性,并受金属盐离子的种类和添加量的影响,在高浓度、低盐、中性pH条件下表观黏度达到最大。本研究表明DGPS具有较好的抗氧化活性和温度稳定性具有在日化和环保等领域的应用潜力。
YS-33,a strain to degrade polycyclic aromatic hydrocarbon,was isolated from the soil of a coking plant.Through physiological,biochemical,and 16S rRNA sequencing,this strain was identified to be the biphenyl degrading bacterium(Dyella ginsengsoli YS-33).The extracellular polysaccharide(DGPS)of the bacterium were obtained by fermentation,which was further analyzed by high performance liquid chromatography(HPLC)and high performance gel permeation chromatography(HPGPC).As a result,DGPS proved to be an acidic polysaccharide composed of glucuronic acid(GlcAc)and galactose(Gal),with a molar ratio of 1∶6.8,and the molecular weight of DGPS was determined to be 1.71×10^(6).Importantly,polysaccharides displayed good antioxidant activity.Based on the subsequent rheological research,DGPS solution exhibited non-Newtonian fluid shear thinning characteristics,with its apparent viscosity in concentration-and temperature-dependent manner.Moreover,the viscosity of DGPS could be affected by the type and amount of metal ions added,with the apparent viscosity reaching its maximum under high concentration,low salt,and neutral pH conditions.Overall,DGPS demonstrated good antioxidant activity and temperature stability,and has potential applications in the fields of daily chemical and environmental protection.
作者
杜玮
孟巧
何跃
章雷
张可桂
刘迎
DU Wei;MENG Qiao;HE Yue;ZHANG Lei;ZHANG Kegui;LIU Ying(Taihu Lake Treatment Co.,Ltd.,Wuxi 214062,China;College of Food Science and Light Industry,Nanjing Tech University,Nanjing 211800,China;Nanjing Institute of Environmental Science,Ministry of Ecology and Environment,Nanjing 210042,China)
出处
《生物加工过程》
2025年第5期521-529,共9页
Chinese Journal of Bioprocess Engineering
基金
生态环境部南京环境科学研究所基本科研业务专项(GYZX220302)。
