摘要
磷是植物生长发育的关键营养元素,但土壤中磷素常以植物难以吸收的形式存在。将芽孢杆菌HJB17衍生的碱性磷酸酶(PB)和生物多糖复合物(BPS)结合制备碱性磷酸酶载体复合物(P-BPS),分析其酶学性质和研究其在土壤中的活性变化。酶学性质表明,P-BPS和PB最适反应温度均为37℃,P-BPS相较于PB在高温下表现出更强的耐受性。P-BPS 55℃时活性基本不受影响,在65℃仍能保持较高活性。此外,还分析了P-BPS对4个地区土壤样品有效磷、3个地区番茄植株生长前后土壤样品有效磷和2个地区番茄植株生长指标的影响。结果表明:(1)4个地区初始有效磷不同的土样中,P-BPS都是在加入样品的0.5%时有效磷增长率最高,河南信阳、山东青岛、广西巴马、广东新会最高分别可达到40.1%、49.5%、48.6%、49.1%;(2)番茄试验田的土壤有效磷也有增长,添加量为0.5%时最高,平谷、北京林业大学校内、青岛试验田增幅分别为27.9%、28.1%、29.0%;(3)在加入有机肥0.5%时,平谷试验田番茄株高、主根长均增幅116%、56%,北京林业大学内试验田分别增幅82%、90%。综上所述,外源性添加P-BPS能够提升土壤有效磷含量,P-BPS的直接应用可以作为土壤磷肥力的可持续来源。
Phosphorus is a critical nutrient for plant growth and development,but it often exists in forms in the soil that are not readily accessible to plants.In this study,an alkaline phosphatase(PB)derived from Bacillus subtilis HJB17 and a biopolysaccharide(BPS)composite were combined to form an alkaline phosphatase-biocarrier complex(P-BPS).The enzymatic properties of P-BPS were evaluated,and its activity in soil was assessed.Enzyme activity analysis revealed that both P-BPS and PB exhibited an optimal reaction temperature of 37℃,with P-BPS demonstrating superior thermal stability compared to PB.At 55℃,the enzyme activity of P-BPS remained largely unaffected,and it still maintained substantial activity at 65℃.Additionally,the effects of P-BPS on the available phosphorus content in soil samples from four regions,as well as on soil samples before and after the growth of tomato plants from three regions,and tomato plant growth indicators in two regions were investigated.The results indicated that:(1)In soil samples from four regions with varying initial levels of available phosphorus,the highest increase in available phosphorus content was observed when P-BPS was added at 0.5%,with maximum increases of 40.1%,49.5%,48.6%and 49.1%in Xinyang Henan,Shandong Qingdao,Guangxi Bama and Guangdong Xinhui,respectively;(2)In tomato experimental fields,soil available phosphorus content also increased,with the highest increments observed at 0.5%addition,showing increases of 27.9%,28.1%and 29.0%in Pinggu,Forestry University,and Qingdao experimental fields,respectively;(3)Regarding tomato plant growth,the addition of 0.5%P-BPS resulted in increases in plant height and main root length of 116%and 56%,respectively,in Pinggu experimental fields,and 82%and 90%,respectively,in Beijing Forestry University experimental fields.In conclusion,the exogenous application of P-BPS effectively enhanced the bioavailability of phosphorus in soil,and its direct use could provide a sustainable source of phosphorus fertility in agricultural soils.
作者
王奔
李江涛
鲁芳
国世阳
王思齐
杨丽红
金靖琳
刘龙
刘海霞
高伟
WANG Ben;LI Jiang-tao;LU Fang;GUO Shi-yang;WANG Si-qi;YANG Li-hong;JIN Jing-lin;LIU Long;LIU Hai-xia;GAO Wei(Biological Physics Laboratory,College of Science,Beijing Forestry University,Beijing 100083)
出处
《中国土壤与肥料》
北大核心
2025年第9期129-137,共9页
Soil and Fertilizer Sciences in China
基金
国家自然科学基金项目(31070651)。
关键词
碱性磷酸酶载体复合物
磷酸酶活性
土壤有效磷
番茄植株生长
alkaline phosphatase carrier complex
phosphatase enzyme activity
soil available phosphorus
tomato plant growth
