摘要
目的:旨在阐明长链非编码RNA MEG3对鼻咽癌细胞恶性生物学行为(增殖、迁移、侵袭及凋亡)的调控作用,并解析其分子机制。方法:通过体外构建鼻咽癌细胞系TW03、CNE-2的MEG3过表达模型,采用qRT-PCR检测基因表达水平,结合CCK-8法、Transwell实验及流式细胞术系统性评估MEG3对细胞增殖、迁移侵袭及凋亡的影响。结果:相较于正常鼻咽上皮细胞NP69,鼻咽癌细胞中MEG3表达显著下调(0.45±0.03 vs 1.00±0.00,P<0.05)。过表达MEG3后,其mRNA水平升高至5.29±0.05(F=398.057,P<0.01),并显著抑制NPC细胞增殖(96h OD值:0.72±0.04 vs 1.25±0.05,F=9.543,P<0.05)、迁移(32.6±3.1 vs 156.3±8.2,F=1336.342,P<0.05)及侵袭能力(28.4±2.9 vs 142.5±7.5,F=1388.434,P<0.05),同时诱导凋亡率升高至8.09±0.11%(F=801.043,P<0.05)。结论:MEG3在鼻咽癌中呈现表达缺失特征,其功能恢复可通过靶向抑制上皮-间质转化(EMT)进程,有效遏制肿瘤细胞的恶性表型,提示MEG3可作为鼻咽癌分子靶向治疗的潜在干预靶点。
Objective:To illustrate the regulatory role of long non-coding RNA MEG3 on malignant biological behaviors(proliferation,migration,invasion,and apoptosis)in nasopharyngeal carcinoma(NPC)cells and to analyze its molecular mechanisms.Methods:MEG3 overexpression models were constructed in NPC cell lines TW03 and CNE-1.qRT-PCR was employed to detect gene expression levels,while CCK-8 assay,Transwell experiments,and flow cytometry were utilized to systematically evaluate the effects of MEG3 on cell proliferation,migration,invasion,and apoptosis.Results:Compared with normal nasopharyngeal epithelial cells NP69,MEG3 expression was significantly downregulated in NPC cells(0.45±0.03 vs 1.00±0.00,P<0.05).Overexpression of MEG3 elevated its mRNA level to 5.29±0.05(F=398.057,P<0.01),and markedly suppressed NPC cell proliferation(96h OD value:0.72±0.04 vs 1.25±0.05,F=9.543,P<0.05),migration(32.6±3.1 vs 156.3±8.2,F=1336.342,P<0.05),and invasion(28.4±2.9 vs 142.5±7.5,F=1388.434,P<0.05),while inducing an increased apoptosis rate of 8.09±0.11%(F=801.043,P<0.05).Conclusion:MEG3 exhibits a loss-of-expression signature in NPC,and its functional restoration effectively restrains malignant phenotypes of tumor cells by targeting the inhibition of epithelial-mesenchymal transition(EMT),suggesting MEG3 as a potential therapeutic target for molecularly targeted therapy in NPC.
作者
包伟晶
宁佳羽
魏日富
陈莉
李玲国
何观文
BAO Weijing;NING Jiayu;WEI Rifu(Ningde Municipal Hospital Affiliated to Ningde Normal University/Ningde Clinical Medical College,Fujian Medical University,Fujian Ningde 352100,China)
出处
《河北医学》
2025年第10期1646-1650,共5页
Hebei Medicine
基金
福建省宁德市自然科学基金联合项目,(编号:2022J15和2024J18)。
关键词
鼻咽癌
调控网络
恶性进展
母系表达基因3
Nasopharyngeal carcinoma
Regulatory network
Malignant progression
Maternally expressed gene 3
