摘要
【目的】探究特基拉芽孢杆菌Bacillus tequilensis不同施用方式(喷叶与灌根)对油茶Camellia oleifera防御反应的诱导机制。【方法】利用对油茶果生炭疽菌有较好抑制作用的特基拉芽孢杆菌B.tequilensis YYC155,通过喷叶和灌根处理诱导油茶苗,测定防御相关酶活性及物质含量,并结合转录组分析其调控机制。【结果】与对照相比,2种处理方式均能提高油茶防御酶活性,包括苯丙氨酸解氨酶(Phenylalanine ammonia-lyase,PAL)、肉桂酸-4-羟化酶(Cinnamate-4-hydroxylase,C4H)、4-香豆酸辅酶A连接酶(4-coumarate coenzyme A ligase,4CL)和查尔酮异构酶(Chalcone isomerase,CHS),并促进总酚和黄酮物质积累。转录组分析显示,喷叶样本(YYC155P)-vs-灌根样本(YYC155G)鉴定出652个差异表达基因(Differentially expressed genes,DEGs),被富集到66条KEGG通路,其中,4条抗氧化系统相关通路中有72.41%的DEGs上调,8条次生代谢相关通路中有47.37%的DEGs上调。【结论】喷叶处理主要通过次生代谢途径激活油茶防御反应,灌根处理则更依赖抗氧化系统的增强。
【Objective】This study aimed to investigate the induction mechanism of defense responses in Camellia oleifera induced by different application methods(leaf spraying and root irrigation)of Bacillus tequilensis.【Method】B.tequilensis YYC155,which exhibits strong inhibitory effects against Colletotrichum fructicola(the causal agent of anthracnose on C.oleifera),was employed to induce defense responses in C.oleifera seedlings through leaf spraying and root irrigation treatments.Subsequently,the activities of defenserelated enzymes and the contents of substance compounds were determined,and transcriptomic analysis was conducted to elucidate the underlying regulatory mechanisms.【Result】Compared with the control group,both treatments significantly enhanced the activities of defense-related enzymes in C.oleifera,including phenylalanine ammonia-lyase(PAL),cinnamate-4-hydroxylase(C4H),4-coumarate coenzyme A ligase(4CL),and chalcone isomerase(CHS).Additionally,these treatments promoted the accumulation of total phenols and flavonoids.Transcriptomic analysis revealed that there were 652 differentially expressed genes(DEGs)identified between leaf spraying samples(YYC155P)and root irrigation samples(YYC155G),which were enriched in 66 KEGG pathways.Among these pathways,72.41%of the DEGs in the four antioxidant systemrelated pathways were upregulated,while 47.37%of the DEGs in the eight secondary metabolism-related pathways were upregulated.【Conclusion】Leaf spraying treatment primarily activates defense responses in C.oleifera through secondary metabolic pathways,whereas root irrigation treatment relies more on the enhancement of the antioxidant system.
作者
彭睿琦
周嫒婷
葛白瑞雪
马焕成
王芳
伍建榕
PENG Ruiqi;ZHOU Aiting;GE Bairuixue;MA Huancheng;WANG Fang;WU Jianrong(College of Forestry,Southwest Forestry University,Kunming 650224,China)
出处
《华南农业大学学报》
北大核心
2025年第6期895-906,共12页
Journal of South China Agricultural University
基金
国家重点研发计划(2019YFD100200X)
国家自然科学基金(31860208,31560207)
云南省农业联合专项(202301BD070001-102)。
关键词
特基拉芽孢杆菌
诱导
防御反应
油茶
Bacillus tequilensis
Induction
Defense response
Camellia oleifera
