摘要
为评估花花柴(Karelinia caspia)种质的耐盐性差异,本研究采用水培方法测定了不同浓度盐处理(0,200,300,400,500 mmol·L^(-1) NaCl)对22份花花柴种质生长及生理指标的影响,并采用主成分分析、隶属函数分析、聚类分析和回归分析对花花柴耐盐性进行综合评价。结果表明:花花柴在不同盐浓度处理下各指标差异较大,需构建多梯度盐胁迫结合多指标的综合评价体系。通过主成分分析和隶属函数分析得出的耐盐综合评价D值,将花花柴种质分为4类:强耐盐型、耐盐型、弱耐盐型、敏盐型。回归分析表明,株高、叶绿素a/b、过氧化物酶活性、最大光化学效率、可溶性糖以及脯氨酸可用做花花柴种质耐盐性评价首选指标。本研究为花花柴耐盐种质的创新利用、耐盐基因的挖掘提供理论依据,同时为盐碱化地区植被恢复的物种选择提供科学支撑。
To evaluate the variances in salt tolerance of Karelinia caspia germplasm resources,twenty-two germplasms of K.caspia were treated with different concentrations of salt(0,200,300,400,500 mmol·L^(-1) NaCl).Sixteen indicators were measured.Principal component analysis,membership function analysis,cluster analysis,and stepwise regression method were used to comprehensively evaluate the salt tolerance of K.caspia.Results showed that there were significant differences in each indicator of K.caspia under different salt treatments,indicating the necessary to construct a comprehensive evaluation system combining multi-gradient salt stress and multiple indicators.Based on the salt tolerance comprehensive evaluation index(D-value)derived from PCA and membership function analysis,the germplasms were classified into four distinct categories:strongly salt-tolerant,moderately salt-tolerant,weakly salt-tolerant,and salt-sensitive types.Regression analysis identified six key indicators for salt tolerance evaluation:plant height,chlorophyll a/b ratio,antioxidant enzyme activity,maximum photochemical efficiency,soluble sugar content,and proline content.This study provided a theoretical foundation for the innovative utilizationn of salt-tolerant K.caspia germplasms and the discovery of salt-resistant genes.It also offerd scientific support for species selection in vegetation restoration projects across salinized regions.
作者
李翠
杨志臣
国宸嘉
郑美
赵春桥
侯新村
郭强
LI Cui;YANG Zhi-chen;GUO Chen-jia;ZHENG Mei;ZHAO Chun-qiao;HOU Xin-cun;GUO Qiang(Institute of Grassland,Flowers and Ecology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China)
出处
《草地学报》
北大核心
2025年第10期3353-3361,共9页
Acta Agrestia Sinica
基金
北京市农林科学院科技创新能力建设专项(KJCX20240409)
国家自然科学基金(32371758)
北京市农林科学院科技创新能力建设专项(KJCX20251303)
北京市农林科学院青年基金(QNJJ202221)
北京市农林科学院科技创新能力建设专项(KJCX20220407)资助。
关键词
花花柴
耐盐性
综合评价
隶属函数分析
回归分析
Karelinia caspia
Salt tolerance
Comprehensive evaluation
Subordinate function analysis
Regression analysis
