摘要
目的 结合数字化设计与三维打印(3DP)技术,制作载左旋紫草素的甘露醇-海藻酸钙敷料,并检测其体外释药及抗菌性能。方法 将梯度浓度50、100、200、400、800、1 600 mg/L的左旋紫草素作为载药组,100μg青霉素组作为对照组,分别负载于40 g/L甘露醇海藻酸钠中,通过3DP技术制备紫草-甘露醇-海藻酸钙网格状水凝胶(15 mm×15 mm×3 mm);显微电镜观察材料横断面三维多孔结构;动态扩散法检测药物释放情况,菌落形成单位计数法及结晶紫染色法检测材料抗菌性能。结果 打印外观与计算机辅助设计模型精度±0.15 mm,边缘几何轮廓清晰;扫描电子显微镜显示具有良好的多孔载药结构;应用体外释药标准曲线显示:释药行为呈现典型缓释特征,无突释及平台期。抗菌性能显著,稀释涂布平板法证实:24 h时200 mg/L敷料组对金黄色葡萄球菌与大肠杆菌实现完全抑菌(抑菌率>99.9%),结晶紫染色显示载药敷料对48 h成熟菌膜有效抑菌率超过战创伤常规药物的抗菌疗效(P<0.01)。结论 通过数字化设计与3DP技术,可以构建载不同药物的特定形状水凝胶,实现特定释药-高效抗菌的功能,未来有望为颌面部战创伤愈合提供新策略。
Objective To fabricate a mannitol-calcium alginate dressing loaded with L-shikonin by integrating digital design and three-dimensional printing(3DP)technology,and to evaluate its in vitro drug release and antibacterial properties.Methods L-shikonin at gradient concentrations of 50,100,200,400,800,and 1600 mg/L was used as the drug loading group,and 100μg penicillin group was used as the control group,which were respectively loaded into 40 g/L mannitol-sodium alginate.A shikonin mannitol calcium alginate grid hydrogel(15 mm×15 mm×3 mm)was prepared using 3DP technology.The cross-sectional three-dimensional porous structure of the material was observed using scanning electron microscopy(SEM).The drug release profile was assessed using the dynamic diffusion method,and the antibacterial properties were evaluated using colony-forming unit counting and crystal violet staining.Results The printed appearance showed a precision of±0.15 mm compared to the computer-aided design model,with clear geometric contours at the edges.SEM revealed a well-structured porous drug-loading matrix.The in vitro drug release standard curve demonstrated a typical sustained-release profile,with no burst release or plateau phase.The antibacterial performance was significant:the dilution plating method confirmed that the 200 mg/L dressing group achieved complete inhibition(inhibition rate>99.9%)against Staphylococcus aureus and Escherichia coli at 24 h.Crystal violet staining indicated that the drug-loaded dressing exhibited an effective inhibition rate against 48-hour mature biofilms that surpassed the antibacterial efficacy of conventional drugs for combat trauma(P<0.01).Conclusion Digital design combined with 3DP technology enables the construction of hydrogels with specific shapes loaded with various drugs,achieving targeted drug release and high-efficiency antibacterial functions.This approach holds promise as a novel strategy for the healing of maxillofacial combat trauma.
作者
单文汐
李恒
曹政
吴江
SHAN Wenxi;LI Heng;CAO Zheng;WU Jiang(State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi Key Laboratory of Stomatology,Department of Dental Materials,School of Stomatology,Air Force Medical University,Xi'an 710032,China;State Key Laboratory of Oral&Maxillofacial Reconstruction and Regeneration,National Clinical Research Center for Oral Diseases,Shaanxi Key Laboratory of Stomatology,Department of Oral and Maxillofacial Surgery,School of Stomatology,Air Force Medical University,Xi'an 710032,China)
出处
《空军军医大学学报》
2025年第10期1300-1305,共6页
Journal of Air Force Medical University
基金
陕西省卫生健康科研创新能力提升计划团队支持项目(2024TD-07)
空军军医大学军事人才孵化军事医学人才项目(2022-fhjsyxrc14)。
关键词
三维打印
左旋紫草素
战创伤
水凝胶敷料
即刻交联
多孔结构
药物释放
抗菌性能
three-dimensional printing
L-shikonin
combat trauma
hydrogel dressings
immediate crosslinking
porous structure
drug release
antibacterial properties
