摘要
为明确转录因子StMYB90-like在花青素合成代谢中的调控机制,以彩色马铃薯黑金刚为研究对象,基于前期生物信息学筛选的转录因子StMYB90-like,通过干扰载体构建、遗传转化、荧光定量分析、花青素含量的测定,对转基因马铃薯进行检测和分析。结果表明,干扰彩色马铃薯黑金刚中转录因子StMYB90-like的表达后,其自身表达量显著降低,且马铃薯叶片中的花青素含量极显著降低了47.94%,马铃薯块茎颜色变浅,马铃薯块茎中的花青素含量显著降低了9.79%。说明转录因子StMYB90-like能够正向调控彩色马铃薯中花青素的合成,推测其可能通过上调花青素生物合成相关结构基因的方式调控马铃薯花青素的合成和积累。
To clarify the regulatory mechanism of the transcription factor StMYB90-like in anthocyanin synthesis and metabolism,this study used the colored potato Heijingang as the material.Based on the previously identified StMYB90-like gene through bioinformatics screening,an interference vector was further constructed and genetically transformed.Through fluorescence quantitative analysis,anthocyanin content determination,and performance observation of transgenic plants,it was found that interference with StMYB90-like expression significantly reduced their transcription levels.At the same time,the anthocyanin content in leaves decreased by 47.94%,the color of tubers became lighter,and the anthocyanin content in tubers decreased by 9.79%.The above results indicate that the gene StMYB90-like positively regulates anthocyanin synthesis in colored potatoes,likely by upregulating the expression of structural genes involved in anthocyanin biosynthesis,thereby modulating the synthesis and accumulation of anthocyanins in potato tissues.
作者
潘阳
王立春
付学鹏
田国奎
王海艳
李凤云
庞泽
李明雪
PAN Yang;WANG Lichun;FU Xuepeng;TIAN Guokui;WANG Haiyan;LI Fengyun;PANG Ze;LI Mingxue(Keshan Branch of Heilongjiang Academy of Agricultural Sciences/Heilongjiang Province Potato Germplasm Resources and Genetic Improvement Engineering Technology Research Center/Key Laboratory of Potato Biology and Genetics Breeding,Ministry of Agriculture and Rural Affairs,Qiqihar 161006,Heilongjiang,China;College of Life Science and Agroforestry,Qiqihar University,Qiqihar 161006,Heilongjiang,China)
出处
《中国瓜菜》
北大核心
2025年第10期56-60,共5页
China Cucurbits And Vegetables
基金
黑龙江省农业科技创新跨越工程农业科技基础创新优青项目(CX23YQ12)
黑龙江省省属科研院所科研业务费资助项目(CZKYF2024-1-C003)
黑龙江省农业科学院克山分院2023年度先导培育项目(XDZDA2023-01)
黑龙江省农业科技创新跨越工程项目(CX23GG02)
黑龙江省“揭榜挂帅”科技攻关项目(2022ZXJ06B02-02a,2022ZXJ06B01-03)
国家马铃薯产业技术体系齐齐哈尔综合试验站项目(CARS-09-ES37)
齐齐哈尔市科技计划联合引导项目(LNYGG-2024005)。
