摘要
为探究野生和栽培两种模式下鸟巢蕨次生代谢物差异,采用超高效液相色谱-四级杆飞行时间串联质谱(UHPLC-QTOF-MS/MS)技术,对原生环境(B组)与栽培环境(C组)鸟巢蕨叶片开展广泛靶向代谢组学分析。通过正交偏最小二乘判别分析(OPLS-DA)筛选差异代谢物,并进行相关性分析和KEGG代谢通路富集分析。结果表明,野生型和栽培型鸟巢蕨共鉴定出代谢物647个,其中,莽草酸及苯丙酸类占比最高(37%),其次为萜类(19%)、生物碱类(16%)等。PCA与OPLS-DA分析结果显示,野生组与栽培组在代谢水平上存在显著分离;共筛选出326种差异代谢物,其中栽培组上调146种,下调180种,主要集中于莽草酸及苯丙酸类、生物碱类和萜类。KEGG通路富集分析显示,共有15条代谢通路显著富集,其中黄酮和黄酮醇生物合成、ABC转运蛋白通路及氨基酸代谢相关通路富集程度最高。研究结果为优化鸟巢蕨栽培条件、提升活性成分含量及药用价值评估提供了代谢组学依据,并为后续解析其代谢调控机制及开发应用奠定了基础。
To investigate the differences in secondary metabolites of Asplenium nidus between wild and cultivated modes,a widely targeted metabolomics analysis was performed on leaf samples of A.nidus from native(Group B)and cultivated(Group C)environments using ultra-high-performance liquid chromatography-quadrupole time-of-flight tandem mass spectrometry(UHPLC-QTOF-MS/MS).Differential metabolites were screened by orthogonal partial least squares discriminant analysis(OPLS-DA),followed by correlation analysis and KEGG metabolic pathway enrichment analysis.The results showed that a total of 647 metabolites were identified in wild and cultivated A.nidus,among which shikimic acid and phenylpropanoids accounted for the highest proportion(37%),followed by terpenoids(19%)and alkaloids(16%).Principal component analysis(PCA)and OPLS-DA results indicated a significant separation in metabolic levels between the wild group and the cultivated group.A total of 326 differential metabolites were screened,including 146 up-regulated and 180 down-regulated,mainly concentrated in shikimales and phenyloropanolds,alkaloids,and terpenoids.KEGG pathway enrichment analysis revealed 15 significantly enriched metabolic pathways,among which flavonoid and flavonol biosynthesis,ABC transporter pathways,and amino acid metabolism-related pathways showed the highest enrichment levels.The findings provide a metabolomic basis for optimizing the cultivation conditions of A.nidus,improving the content of active components,and evaluating its medicinal value,and also lay a foundation for subsequent exploration of its metabolic regulation mechanisms and development applications.
作者
赵王瑞
毕建祥
董诗浪
黄文龙
张才安
王步天
谢纯
曲鹏
葛宇
杜华波
周春燕
ZHAO Wangrui;BI Jianxiang;DONG Shilang;HUANG Wenlong;ZHANG Caian;WANG Butian;XIE Chun;QU Peng;GE Yu;DU Huabo;ZHOU Chunyan(College of Tropical Crops,Yunnan Agricultural University,Puer 665099,Yunnan,China;Jinghong Seed Industry Development Center,Jinghong 666106,Yunnan,China)
出处
《中国瓜菜》
北大核心
2025年第10期48-55,共8页
China Cucurbits And Vegetables
基金
国家天然橡胶产业技术体系普洱综合试验站(CARS-33-YN4)
云南省黄华孙专家工作站(202305AF150125)
孟连县农民院士科技服务站
云南农业大学本科教学改革工程项目(YNAUJG2024010)
云南农业大学热带作物学院教育教学改革研究项目(202405)。
关键词
鸟巢蕨
次生代谢组
正交偏最小二乘判别分析
人工栽培
Asplenium nidus
Secondary metabolome
Orthogonal partial least squares discriminant analysis
Cultivated artificially
