摘要
基因编辑小鼠模型作为现代生物医学研究的核心工具,在疾病机制解析、药物研发及基因功能研究等领域发挥着不可替代的作用。然而,高校在基因编辑小鼠的制备、饲养和数据分析过程中常面临技术复杂、资源分散、管理效率低下等挑战。为解决上述问题,构建整合全链条技术服务与智能化管理体系的基因编辑小鼠一站式服务平台,已成为高校实验动物中心突破现有困境的必然选择。该平台须具备基因编辑小鼠构建、繁殖扩群、生物净化、品系保种、濒危品系拯救等核心功能,形成覆盖“靶点设计-模型构建-种群扩繁-遗传质量控制-生物样本保存”的全流程技术服务体系,以促进高校实验动物中心从提供“单一技术服务”向提供“全流程解决方案”的战略转型。以上海交通大学实验动物中心为例,该中心通过技术优化解决了显微操作成功率低与基因型鉴定缺乏标准化操作流程等技术缺陷,通过可追溯性管理和信息共享解决了课题组小鼠品系管理混乱与重复构建造成的科研经费浪费等问题,通过系统性整合与优化技术服务解决了以往服务体系碎片化可能导致的效率低下、资源浪费、协作困难等问题。本文从集约化管理、服务能力建设和支撑体系建设三个方面,系统探讨了基因编辑小鼠服务平台的建设路径与实践经验,以期为高校同类平台的建设提供可借鉴的管理模式和实施路径。
Gene-edited mouse models,as a core tool in modern biomedical research,play an irreplaceable role in fields such as disease mechanism analysis,drug development,and gene function studies.However,universities often face challenges including technical complexity,dispersed resources,and low management efficiency during the preparation,breeding,and data analysis of gene-edited mice.To address these issues,establishing a one-stop service platform for gene-edited mice that integrates fullchain technical services and an intelligent management system has become an inevitable choice for university laboratory animal centers to break through current difficulties.This platform must possess core functions including gene-edited mouse construction,breeding and colony expansion,biological purification,strain preservation,and endangered strain rescue,forming a whole-process technical service system covering"target design-model construction-population expansion-genetic quality control-biological sample preservation",to promote the strategic transformation of university laboratory animal centers from providing"single technical services"to providing"whole-process solutions".Taking the Laboratory Animal Center of Shanghai Jiao Tong University as an example,through technical optimization,the technical defects such as low microoperation success rate and insufficient standardization of genotype identification were solved.Through traceability management and information sharing,problems such as chaotic management of mouse strains in research groups and waste of research funds caused by repeated constructions were solved.Through systematic integration and optimization of technical services,problems such as low efficiency,resource waste,and collaboration difficulties that may be caused by fragmented service systems were solved.This paper systematically discusses the construction path and practical experience of the gene-edited mouse service platform from three perspectives:intensive management,service capacity building,and support system construction,aiming to provide referable management models and implementation paths for the construction of similar platforms in other universities.
作者
聂永强
王朝霞
NIE Yongqiang;WANG Zhaoxia(Laboratory Animal Center of Shanghai Jiao Tong University,Shanghai 200240,China)
出处
《实验动物与比较医学》
2025年第5期642-648,共7页
Laboratory Animal and Comparative Medicine
关键词
基因编辑小鼠
一站式服务平台
高校实验动物中心
集约化管理
全流程技术服务体系
Gene-edited mice
One-stop service platform
University laboratory animal center
Intensive management
Whole-process technical service system
