摘要
转基因作物包括抗虫棉已经在世界广泛种植,抗虫棉的推广带来了显著的经济效益和社会效益。棉花是世界上最主要的天然纤维来源和最重要的经济作物之一。农杆菌介导的遗传转化技术,结合体细胞胚胎发生和器官再生途径,是获取转基因植物的主要方法。棉花是通过体细胞胚胎发生途径实现植株再生的典型作物。通过体细胞胚胎发生获得再生植株的过程,不仅是现代生物技术在棉花领域的重要应用,而且对于推动棉花遗传改良和品种创新具有重要意义。棉花体细胞胚胎发生过程复杂,调控机制被广泛研究。然而基因型限制和再生效率低仍然是制约棉花遗传转化的主要瓶颈。本文对棉花体细胞胚胎发生的研究进展进行了概述,总结了棉花体细胞胚胎发生的研究现状、棉花体细胞胚胎发生的主要影响因素和分子机制研究进展,并对今后棉花体细胞胚胎发生研究进行了展望。
Transgenic crops,including insect-resistant cotton,have been widely cultivated globally,yielding significant economic and social benefits.Cotton is the foremost natural fiber source and one of the most important cash crops worldwide.The genetic transformation technology mediated by Agrobacterium tumefaciens,based on somatic embryogenesis or organogenesis,has become the primary method for obtaining transgenic plants.Particularly for cotton,a crop that typically regenerates through somatic embryogenesis,this process is not only crucial for the application of modern biotechnology in cotton,but also plays a vital role in genetic improvement and variety innovation in cotton.However,cotton somatic embryogenesis is a complex process;although its regulatory mechanisms have been extensively studied,genotype-dependence and low regeneration efficiency remain significant challenges in cotton genetic transformation.This article provides an overview of the research progress in cotton somatic embryogenesis,summarizing the status of the cotton somatic embryogenesis system,including key influencing factors and molecular mechanisms,and offers perspectives on future research in this area.
作者
刘琳琳
甄军波
刘迪
封丛华
迟吉娜
Liu Linlin;Zhen Junbo;Liu Di;Feng Conghua;Chi Jina(Cotton Research Institute,Hebei Academy of Agriculture and Forestry Sciences/Key Laboratory of Cotton Biology and Genetic Breeding in Huanghuaihai Semi-arid Region,Ministry of Agriculture and Rural Affairs,Shijiazhuang 050051,China)
出处
《棉花学报》
北大核心
2025年第4期306-320,共15页
Cotton Science
基金
河北省农林科学院现代农业科技创新工程专项课题(2022KJCXZX-MHS-7)
河北省棉花产业技术体系(HBCT2024100204)。
关键词
棉花
体细胞胚胎发生
组织培养
分子机制
cotton
somatic embryogenesis
tissue culture
molecular mechanism
