摘要
稻瘟病是水稻生产中最严重的真菌病害。高氮肥施用容易引发水稻感稻瘟病,但相关遗传基础尚不清楚。为解析氮素吸收利用途径在稻瘟菌侵染过程中的作用,本研究利用实时荧光定量PCR方法对稻瘟菌侵染后水稻氮素吸收、代谢和利用信号途径相关基因的表达模式进行分析。结果显示,在分析的33个相关基因中,有22个基因(包括8个硝酸盐转运蛋白基因、3个铵盐转运蛋白基因、3个氮素同化相关基因、5个氮信号调控相关基因和3个氮素利用相关基因)在接种稻瘟菌亲和小种后4 h受到激活显著上调表达,而在侵染中后期(8~96 h),其表达水平与对照相比无显著变化;有28个基因(包括11个硝酸盐转运蛋白基因、4个铵盐转运蛋白基因、5个氮素同化相关基因、5个氮信号调控相关基因和3个氮素利用相关基因)在接种稻瘟菌非亲和小种后(4~96 h)受抑制下调表达。这些结果表明,氮素吸收、代谢和利用信号途径相关基因的激活在促进早期稻瘟菌侵染过程中发挥着重要的作用,可为进一步解析氮素促进水稻感病的分子机制提供参考。
Rice blast is the most devastating fungal disease in rice production.High nitrogen fertilizer application tends to promote rice susceptibility to blast,however,the underlying genetic basis remains unclear.To elucidate the role of nitrogen uptake and utilization pathways during Magnaporthe oryzae infection,in this study,the expression patterns of genes associated with rice nitrogen absorption,metabolism and utilization signaling pathways were analyzed by real-time RT-qPCR after blast inoculation.The results showed that among the 33 genes regulating nitrogen uptake and utilization,22 genes including 8 nitrate transporter genes,3 ammonium transporter genes,3 nitrogen assimilation-related genes,5 nitrogen signaling regulatory genes,and 3 nitrogen utilization-related genes were significantly up-regulated at 4 hours after infected by a compatible M.oryzaerace,which showed no significant changes compared to the control during the middle to late infection stages(8~96 h);28 genes including 11 nitrate transporter genes,4 ammonium transporter genes,5 nitrogen assimilation-related genes,5 nitrogen signaling regulatory genes,and 3 nitrogen utilization-related genes were down-regulated upon inoculation with an incompatible M.oryzae race(4~96 h).These findings demonstrated that the activation of nitrogen absorption,metabolism,and utilization signaling pathways played critical roles in facilitating early M.oryzae infection,which could provide references for further unraveling the molecular mechanisms of nitrogen-induced susceptibility to rice blast.
作者
黄浩
林晋军
陈文凤
姚威
陈倩茹
黄希来
吴俊
刘雄伦
刘金灵
Huang Hao;Lin Jinjun;Chen Wenfeng;Yao Wei;Chen Qianru;Huang Xilai;Wu Jun;Liu Xionglun;Liu Jinling(College of Agronomy,Hunan Agricultural University/Southern Regional Collaborative Innovation Center for Grain and Oil Crops in China/Hunan Provincial Key Laboratory of Crop Gene Engineering,Changsha 410128,China)
出处
《山东农业科学》
北大核心
2025年第9期31-40,共10页
Shandong Agricultural Sciences
基金
国家自然科学基金项目(31972256)
湖南省2023年度十大技术攻关项目(2023NK1010)。
关键词
水稻
稻瘟病
氮素促进感病
氮素吸收利用信号途径
基因表达
Rice
Rice blast
Nitrogen-induced susceptibility(NIS)
Nitrogen absorption and utiliza-tion signaling pathway
Gene expression
