摘要
目的:探讨青蒿素(ART)减轻不同菌种所致脓毒症小鼠心肌损伤的作用及其可能机制。方法:104只C57/BL小鼠随机分为假手术组(sham组)、革兰氏阳/阴性脓毒症组(G+/G-sepsis组)、革兰氏阳/阴性脓毒症+ART不同剂量组(ART 5、10、15、20、25+G+/G-sepsis)。造模后12 h取样,H&E染色观察心肌病理变化并评分,ELISA法测定小鼠血清CK-MB、cTnⅠ浓度。以ART 25 mg/kg剂量组探讨其减轻脓毒症小鼠心肌损伤的作用机制,心脏超声检测小鼠心脏LVEF、LVFS、LVEDD、LVESD、EDV、ESV水平,透射电镜观察心肌线粒体损伤并评分。结果:与sham组相比,G+/G-sepsis组心肌损伤和炎症细胞浸润严重、CK-MB、cTnⅠ明显升高,差异均有统计学意义(P<0.05);ART各剂量组显著减轻心肌纤维损伤和炎症细胞浸润,损伤评分、血清CK-MB、cTnⅠ水平较G+/G-sepsis组呈浓度依赖性下降,差异均有统计学意义(P<0.05);与sham组相比,G+/G-sepsis组心肌收缩功能下降、心肌线粒体呈严重的形态损伤及评分,差异有统计学意义(P<0.05);与G+/G-sepsis组相比,ART 25+G+/G-sepsis组心肌收缩功能得到改善且评分下降、仅少部分线粒体形态破坏,差异有统计学意义(P<0.05)。结论:青蒿素对金黄色葡萄球菌/大肠埃希菌所致脓毒症小鼠心肌损伤有保护作用,其机制可能与改善心肌线粒体功能相关。
Objective:To explore the effect of artemisinin(ART)in reducing myocardial injury in mice caused by different strains.Methods:Atotal of 104 C57/BL mice were randomized to the sham surgery group(sham group),the Gram positive/negative sepsis group(G+/G‐sepsis group),and the Gram positive/negative sepsis+ART group(ART 5、10、15、20、25+G+/G−sepsis).Take samples 12 h after molding,myocardial pathological changes were observed and scored by H&E staining,and mouse serum CK‐MB and cTnⅠconcentrations were determined by ELISA.The mechanism of ART in alleviating myocardial injury in septic mice was explored in the ART 25 mg/kg dose group.The LVEF,LVFS,LVEDD,LVESD,EDV and ESV levels of the mice hearts were detected by cardiac ultrasound,and the myocardial mitochondrial damage was observed and scored by transmission electron microscopy.Results:Compared to the sham group,the G+/G−sepsis group had severe myocardial injury and inflammatory cell infiltration,CK‐MB and cTnⅠ,which were significant(P<0.05).Each dose group of ART significantly reduced cardiac muscle fiber injury and inflammatory cell infiltration,and the injury score,serum CK‐MB and cTnⅠlevels showed a concentration‐dependent significantly decrease compared to G+/G−sepsis group(P<0.05);compared to the sham group,the G+/G−sepsis group showed decreased myocardial systolic function,severe myocardial mitochondria,and significant morphological damage and score(P<0.05);Compared to the G+/G−sepsis group,the ART 25+G+/Gsepsis group had improved myocardial systolic function and mitochondrial damage score decreased scores,and only a small part of the mitochondrial morphology was disrupted,showing a statistically significant difference(P<0.05).Conclusion:Artemisinin has a protective effect on myocardial injury in Staphylococcus aureus/Escherichia coli‐induced sepsis in mice,and the mechanism may be related to improve myocardial mitochondrial function.
作者
张琪
赵长甲
姜波
薛庆
许航
任珊
ZHANG Qi;ZHAO Changjia;JIANG Bo;XUE Qing;XU Hang;REN Shan(The First Affiliated Hospital of Shihezi University,Shihezi 832008,China;Xinjiang Production and Construction Corps Critical Care Medicine Professional Quality Control Center,Shihezi 832008,China;Department of Intensive Care Unit,the First Affliated Hospital of Shihezi University,Shihezi 832008,China)
出处
《海南医科大学学报》
北大核心
2025年第19期1469-1476,共8页
Journal of Hainan Medical University
基金
八师石河子市中青年科技创新领军人才计划项目(2020RC001)
中央引导地方科技-新疆生产建设兵团危重症疾病临床医学研究中心(兵科发2022-6)
兵团指导性项目(2023ZD020,2023ZD003)。
关键词
脓毒症
心肌损伤
青蒿素
心肌线粒体
Sepsis
Myocardial injury
Artemisinin
Myocardial mitochondria
