摘要
采用MiSeq高通量测序和纯培养联用技术对采集自江西省南昌市的药曲样品进行微生物多样性解析,以揭示药曲中的微生物群落结构。结果表明:药曲中细菌类群的丰富度和多样性均明显高于真菌类群,细菌主要优势属为片球菌属(Pediococcus)和促生乳杆菌属(Levilactobacillus),平均相对含量分别为38.04%和13.00%,真菌优势属为假丝酵母属(Candida)和复膜孢酵母属(Saccharomycopsis),平均相对含量分别为78.30%和17.92%。从药曲中分离出的9株细菌被鉴定为戊糖片球菌(Pediococcus pentosaceus)、乳酸片球菌(Pediococcus acidilactici)和地衣芽孢杆菌(Bacillus licheniformis)3个种,7株真菌被鉴定为扣囊复膜酵母(Saccharomycopsis fibuligera)、马兰加酵母(Saccharomycopsis malanga)、库德里阿兹威毕赤酵母(Pichia kudriavzevii)和酿酒酵母(Saccharomyces cerevisiae)4个种。
MiSeq high-throughput sequencing and pure culture techniques were combined to analyze the microbial diversity of medicinal koji samples collected from Nanchang city,Jiangxi province.The aim was to reveal the microbial community structure within the koji.The results showed that the richness and diversity of bacterial taxa in the medicinal koji were significantly higher than those of the fungi.The dominant bacterial genera were Pediococcus and Levilactobacillus,with average relative abundances of 38.04%and 13.00%,respectively.The dominant fungal genera were Candida and S accharomycopsis,with average relative abundances of 78.30%and 17.92%,respectively.Nine strains of bacteria isolated from medicinal koji were identified as three species:Pediococcus pentosaceus,Pediococcus acidilactici,and Bacillus licheniformis.Seven strains of fungi were identified as four species:Saccharomycopsis fibuligera,Saccharomycopsis malanga,Pichia kudriavzevii,and Saccharomyces cerevisiae.
作者
黄怡
蔡文超
余培荣
陈炜
单春会
郭壮
王玉荣
`HUANG Yi;CAI Wenchao;YU Peirong;CHEN Wei;SHAN Chunhui;GUO Zhuang;WANG Yurong(Brewing Technology Industrial College,Hubei University of Arts and Sciences,Xiangyang 441053,China;Hubei Provincial Engineering and Technology Research Center for Food Ingredients,Hubei University of Arts and Science,Xiangyang 441053,China;School of Food Science and Technology,Shihezi University,Shihezi 832003,China;Xinjiang Wuwu Liquor Industry Co.,Ltd.,Huyanghe 830099,China)
出处
《轻工学报》
北大核心
2025年第5期29-36,共8页
Journal of Light Industry
基金
新疆生产建设兵团第七师胡杨河市“揭榜挂帅”项目(QS2024011)
湖北省高等学校优秀中青年科技创新团队计划项目(T2023022)。
关键词
药曲
MiSeq高通量测序
纯培养
微生物多样性
medicinal koji
MiSeq high-throughput sequencing
pure culture
microbial diversity
