摘要
目的:探讨X连锁RNA结合蛋白(RBMX)对食管癌Eca109细胞生物学行为的影响,进一步阐明其可能的作用机制。方法:将对数生长期的Eca109细胞分为对照组和实验组,利用Lipofectamine^(TM) 3000转染RBMX特异性小干扰RNA(siRNA)及过表达载体,分别用逆转录实时荧光定量PCR(RT-qPCR)和Western blot法检测各细胞组中RBMX的mRNA和蛋白表达水平。采用CCK-8法、Transwell小室实验、Western blot法分别检测转染后细胞增殖能力、迁移能力及EMT标志分子蛋白表达的变化。结果:与对照组比较,转染RBMX siRNA后,Eca109细胞中RBMX的mRNA和蛋白表达水平明显降低(P<0.05);转染过表达载体后,细胞中RBMX的mRNA和蛋白表达水平明显升高(P<0.05)。siRNA转染Eca109细胞24 h后,细胞的增殖、迁移能力明显减弱(P<0.05),E-cadherin的蛋白表达水平升高而N-cadherin和vimentin的蛋白表达水平降低(P<0.05);转染过表达载体48 h后,Eca109细胞的增殖、迁移能力明显增强(P<0.05),E-cadherin的蛋白表达水平下降但N-cadherin和vimentin的蛋白表达水平升高(P<0.05)。结论:RBMX能影响Eca109细胞的增殖和迁移能力,并可能通过影响EMT过程参与食管癌的发展。
OBJECTIVE:To investigate mechanisms of RNA-binding motif protein X-linked(RBMX)on biological behavior of esophageal cancer cells in vitro.METHODS:Logarithmic Eca109 cells in culture were divided into control and experimental groups.Lipofectamine^(TM) 3000 was used to transfect small interfering RNA(siRNA)and overexpression vector of RBMX.The mRNA and protein expression levels of RBMX were detected by RT-qPCR and Western blot,respectively.CCK-8,Transwell and Western blot were used to detect changes of cell proliferation,migration and EMT marker protein expression after transfection.RESULTS:Following transfection with RBMX siRNA,the mRNA and protein expression levels of RBMX in Eca109 cells were markedly reduced compared to the control group(P<0.05).Conversely,transfection with the overexpression vector resulted in a significant increase in RBMX mRNA and protein expression levels(P<0.05).After 24 hours of siRNA transfection,proliferation and migration of Eca109 cells were significantly inhibited(P<0.05),accompanied by an increase in E-cadherin protein expression and a decrease in N-cadherin and Vimentin protein expression(P<0.05).In contrast,48 hours post-transfection with the overexpression vector,proliferative and migratory capabilities of Eca109 cells were notably enhanced(P<0.05),with decreased E-cadherin protein expression and increased N-cadherin and Vimentin protein expression(P<0.05).CONCLUSION:RBMX influenced the proliferation and migration of Eca109 cells in vitro,potentially contributing to esophageal cancer development through its impact on the epithelial-mesenchymal transition process.
作者
海妮萨依姆·图尔荪
张志强
刘玲
张琪琪
李卉
HAINISAYIM·Tursun;ZHANG Zhiqiang;LIU Ling;ZHANG Qiqi;LI Hui(Department of Biochemistry and Molecular Biology,School of Basic Medicine,Xinjiang Medical University,Urumqi 830054;The Second Department of Gastroenterology,the First Affiliated Hospital,Xinjiang Medical University,Urumqi 830054,Xinjiang,China)
出处
《癌变·畸变·突变》
2025年第5期405-410,共6页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
新疆维吾尔自治区科技创新团队(天山创新团队)项目(2022TSYCTD0018)。
关键词
食管鳞癌
X连锁RNA结合蛋白
小干扰RNA
上皮间质转化
esophageal squamous cell carcinoma
RNA binding motif protein X-linked
siRNA
epithelialmesenchymal transition
