摘要
目的:探讨不同全血接种量及培养体系对核质桥本底水平和辐射诱导的核质桥水平的影响,为核质桥作为辐射生物剂量计的研究提供科学依据。方法:分别用0、2 Gy ^(60)Coγ射线照射人离体静脉血和指尖血,剂量率为1.0 Gy/min,分别取400、300、200、100、50μL静脉血接种到1或2 mL培养基中,取100μL指尖血接种到1 mL培养基中,应用胞质分裂阻滞法制备核质桥标本,分析单核、双核和多核细胞比例,以及各组核质桥率和微核率。结果:未照射对照组(0 Gy)中,不同静脉血接种量组分析了1048~9768个双核细胞,核质桥率随全血接种量无明显变化规律(P>0.05);50μL组微核率高于其余3组(U=3.61,P<0.01)。2 Gy照射组中,50μL组未能观察到可供分析的细胞,其余4组分析了1569~4400个双核细胞,每细胞的核质桥数为0.009~0.013个,微核数为0.317~0.337个,随全血接种量无明显变化规律(P>0.05)。2 Gy照射组的核质桥率和微核率较未照射对照组显著上升(U=4.24~34.48,P<0.05)。指尖血培养未能观察到可供分析的细胞。结论:采用最低50μL及100μL外周血培养结合1 mL培养基的方案,可分别用于核质桥本底水平及辐射诱导的核质桥水平的研究,指尖血培养用于核质桥分析的可行性尚需进一步研究。
OBJECTIVE:To investigate effects of whole blood inoculation and culture systems on formation of nucleoplasmic bridges(NPB)and on formation of radiation-induced NPB,for providing scientific basis for radiation biological dosimeters.METHODS:Human venous blood and fingertip blood were cultured as follows:400,300,200,100 and 50μL of venous blood were inoculated into 1 or 2 mL medium,100μL of fingertip blood were inoculated into 1 mL medium.These cultures were irradiated with 0 Gy(control group)and 2 Gy ^(60)Coγradiation at a dose rate of 1.0 Gy/min.NPB specimens were prepared by cytokinesis-block method.The proportion of mononucleated,binucleated and multinucleated cells,as well as the NPB and micronucleus(MN)frequencies of each group were analyzed.RESULTS:In the 0 Gy group,1048~9768 binucleated cells were analyzed in different blood inoculation groups.The NPB frequency did not change significantly with the whole blood inoculation(P>0.05).The MN frequency in the 50μL group was higher than that in the other three groups(U=3.61,P<0.01).In the 2 Gy group,there was no cell available for analysis in the 50μL group,and 1569-4400 binucleated cells were analyzed in other blood inoculation groups.The NPB frequency was 0.009-0.013 cells/cell,and the MN frequency was 0.317-0.337 cells/cell,and there was no significant difference with the whole blood inoculum(P>0.05).The NPB and MN frequencies of the 2 Gy group were significantly higher than those of the 0 Gy group(U=4.24-34.48,P<0.05).Fingertip blood culture failed to observe cells for analysis.CONCLUSION:The minimum 50μL and 100μL peripheral blood culture combined with 1 mL culture medium can be used to study the background level of the nucleoplasmic bridge and the radiation-induced nucleoplasmic bridge level respectively.The feasibility of fingertip blood culture for nucleoplasmic bridge analysis still needs further study.
作者
赵骅
陆雪
蔡恬静
齐雪松
刘青杰
ZHAO Hua;LU Xue;CAI Tianjing;QI Xuesong;LIU Qingjie(China CDC Key Laboratory of Radiological Protection and Nuclear Emergency,National Institute for Radiological Protection,Chinese Center for Disease Control and Prevention,Beijing 100088,China)
出处
《癌变·畸变·突变》
2025年第5期400-404,共5页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金(82003393)
中国疾病预防控制中心辐射安全所青年科学研究所长基金(2023-02)。
关键词
核质桥
全血接种量
胞质分裂阻滞
微核
nucleoplasmic bridge
whole blood inoculum
cytokinesis-block
micronucleus
