摘要
[目的]探究黄芩渣有机肥对马铃薯连作土壤基础特性和微生物群落多样性与功能的影响,可为黄芩渣资源化利用和连作马铃薯土壤改良提供理论参考。[方法]以大宗药材黄芩渣和羊粪(质量比为1∶3)为基料,分别添加3%、5%和10%功能菌剂后通过发酵制备得到3种有机肥体系(T1、T2、T3),有机肥施入土壤后相应处理分别为F1、F2、F3。结合田间原位控制试验,解析3种黄芩渣有机肥体系对连作马铃薯农田土壤化学特性、细菌多样性和功能的影响。[结果] 1)与对照组相比,F1~F3处理均能显著提高成熟期土壤速效磷(AP)和速效钾(AK)质量分数,分别提高8.12~12.00、65.63~88.36 mg/kg。2)F1和F2处理均提高马铃薯薯表土细菌菌群总数量,提高幅度分别为60.96%和23.19%,且富集鞘氨醇单胞菌属、假黄色单胞菌属和黄杆菌属等有益菌属。3)LEfSe分析结果显示,施入黄芩渣有机肥后抑制土壤中果胶杆菌属等致病细菌的绝对丰度,富集更多绝对丰度显著高的促进土壤元素循环的有益细菌群落。4)F1~F3处理均能改变土壤细菌介导的碳氮循环功能,提高土壤菌群对有机质的分解潜力和土壤氮素有效性。[结论]施用黄芩渣有机肥能够提高土壤速效磷钾养分质量分数、有益菌属丰度和细菌功能活性,T1处理为最佳有机肥体系。
[Objective]This study aimed to explore the effects of Scutellaria organic fertilizer on the basic characteristics and microbial community diversity and function of soil under continuous cropping of potato can provide theoretical references for the resource utilization of Scutellaria residue and the improvement of continuous potato cropping soils.[Methods]Using the residue of the medicinal herb Scutellaria baicalensis and sheep manure(with a mass ratio of 1∶3)as the base materials,three organic fertilizer systems(labeled as T1,T2,and T3 in sequence)were prepared by fermentation after adding 3%,5%,and 10%functional microbial agents respectively.The corresponding treatments after the organic fertilizer was applied to the soil were labeled as F1,F2,and F3,respectively.The effects of the three Scutellaria residue organic fertilizer systems on soil chemical properties,bacterial diversity and function of continuous potato cropping fields were analyzed with the in-situ control trial.[Results]1)Compared with the control group,all three Scutellaria residue organic fertilizer systems significantly increased the available phosphorus and available potassium contents of the soil at the maturity stage,with increases of 8.12~12.00 mg/kg and 65.63~88.36 mg/kg,respectively.2)The total population of bacterial flora in potato surface soil was increased by 60.96%and 23.19%in F1 and F2 treatments,and enriched with beneficial genera such as Sphingomonas,Pseudoxanthomonas,and Flavobacterium.3)LEfSe analysis showed that the application of Scutellaria residue organic fertilizers suppressed the absolute abundance of pathogenic bacteria such as Pectobacterium in the soil,and enriched more beneficial bacterial communities with significantly high absolute abundance that promote soil element cycling.4)The three kinds of Scutellaria residue organic fertilizer systems altered the function of soil bacterial mediated carbon and nitrogen cycling,and increased the organic matter decomposition potential of soil flora and soil nitrogen availability.[Conclusion]The application of Scutellaria residue organic fertilizer can improve soil available phosphorus and potassium nutrient content,the abundance of beneficial bacterial genera,and bacterial functional activity.The system T1 is the best Scutellaria residue organic fertilizer system.
作者
杜晨璐
却淑涵
焦硕
韦革宏
王红雷
DU Chenlu;QUE Shuhan;JIAO Shuo;WEI Gehong;WANG Honglei(College of Soil and Water Conservation Science and Engineering,Northwest A&F University,Yangling,Shaanxi 712100,China;College of Life Sciences,Northwest A&F University,State Key Laboratory for Crop Stress Resistance and High-Efficiency Production,Shaanxi Key Laboratory of Agriculture and Environmental Microbiology,Yangling,Shaanxi 712100,China;Institute of Soil and Water Conservation,Chinese Academy of Sciences,Ministry of Water Resources,Yangling,Shaanxi 712100,China)
出处
《水土保持学报》
北大核心
2025年第5期400-410,共11页
Journal of Soil and Water Conservation
基金
国家重点研发计划青年科学家项目(2021YFD1900500)。
关键词
中药渣有机肥
连作土壤
土壤微生物
细菌多样性与功能
herb residue organic fertilizer
continuous cropping soil
soil microorganisms
bacterial diversity and function
