摘要
目的:探讨慢性萎缩性胃炎(Chronic atrophic gastritis,CAG)患者血清醌氧化还原酶1(Quinone oxidoreductase 1,NQO1)、晚期氧化蛋白终末产物(Advanced oxidized protein end product,AOPP)水平及与幽门螺杆菌(Helicobacter pylori,Hp)感染、病情严重性的关系。方法:选取2022年7月至2024年7月本院收治的64例CAG患者,根据患者有无幽门螺旋杆菌(Hp)感染分为感染组(n=37)、未感染组(n=27)。采用酶联免疫吸附法检测血清NQO1、AOPP水平,对比两组血清NQO1、AOPP水平,采用Spearman相关分析探讨CAG患者血清NQO1、AOPP与Hp感染的关系。根据CAG患者病情严重程度将患者分为轻中度组(n=29)、重度组(n=35)。对比两组血清NQO1、AOPP水平,采用受试者工作特性(Receiver operating characteristic curve,ROC)曲线评估血清NQO1、AOPP对CAG患者病情严重性的预测价值,采用多因素logistic回归分析CAG患者病情的影响因素。结果:未感染组的血清NQO1水平显著高于感染组,AOPP水平显著低于感染组(P<0.05)。CAG患者血清NQO1与Hp感染呈负相关、血清AOPP与Hp感染呈正相关(r=-0.492、0.573,P<0.001、0.001)。轻中度组的饮酒史、饮食习惯辛辣人数比例、血清AOPP水平均显著低于重度组,血清NQO1水平显著高于重度组(P<0.05)。二分类Logistic逐步回归分析结果显示,饮酒史(OR=2.606,95%CI:1.398~4.861)、饮食习惯辛辣(OR=3.428,95%CI:1.302~9.027)、血清NQO1<4.74μg·L^(-1)(OR=6.527,95%CI:2.333~18.265),血清AOPP≥83.55μmol·L^(-1)(OR=8.240,95%CI:2.628~25.834)是影响CAG患者病情严重性的危险因素(P<0.05)。结论:血清NQO1低表达,AOPP高表达均与感染Hp的CAG患者有关,且是影响CAG病情严重性的独立危险因素,可作为评估CAG病情的潜在生物标志物。
Objective:To investigate serum Quinone oxidoreductase 1(NQO1)and advanced oxidized protein end product(AOPP)levelin in patients with Chronic atrophic gastritis(CAG)and its relationship with Helicobacter pylori(Hp)infection and disease severity.Methods:64 patients with CAG admitted to our hospital from July 2022 to July 2024 were selected as the subjects of this study.CAG patients were divided into infected group(n=37)and uninfected group(n=27)according to whether they were infected with Helicobacter pylori(Hp).Serum NQO1 and AOPP levels were detected by enzyme-related immunosorbent assay,and the serum NQO1 and AOPP levels were compared between the two groups.Spearman correlation analysis was used to explore the relationship between serum NQO1 and AOPP and Hp infection in CAG patients.According to the severity of CAG patients,the patients were divided into mild to moderate group(n=29)and severe group(n=35).Serum NQO1 and AOPP levels of the two groups were compared,and the predictive value of serum NQO1 and AOPP on the severity of CAG patients was evaluated using the Receiver operating characteristic curve(ROC)curve.Multivariate logistic regression was used to analyze the influencing factors of CAG patients.Results:The serum NQO1 level in uninfected group was significantly higher than that in infected group,and the serum AOPP level was significantly lower than that in infected group(P<0.05).In CAG patients,serum NQO1 was negatively correlated with Hp infection,and serum AOPP was positively correlated with Hp infection(r=-0.492,0.573,P<0.001,0.001).The drinking history,proportion of spicy diet and serum AOPP level in the mild to moderate group were significantly lower than those in the severe group,and the serum NQO1 level was significantly higher than that in the severe group(P<0.05).Binary Logistic regression analysis showed that drinking history(OR=2.606,95%CI:1.398-4.861),spicy diet(OR=3.428,95%CI:1.302-9.027),serum NQO1<4.74μg·L^(-1)(OR=6.527,95%CI:2.333-18.265)and serum AOPP≥83.55μmol·L^(-1)(OR=8.240,95%CI:2.628-25.834)were risk factors for the severity of CAG patients(P<0.05).Conclusion:Low expression of serum NQO1 and high expression of AOPP are related to CAG patients infected with Hp,and they are independent risk factors affecting the severity of CAG disease,and can be used as potential biomarkers for evaluating CAG disease.
作者
吴倩
朱冰阳
杜翠翠
Wu Qian;Zhu Bing-yang;Du Cui-cui(Department of Gastroenterology,The First Affiliated Hospital of Nanyang Medical College,Nanyang 473000,Henan,China)
