摘要
该研究构建了Fe_(3)O_(4)@ZIF-8@HRP@SiO_(2)纳米复合材料,提高了辣根过氧化物酶(HRP)的负载效率和稳定性,并用于牡蛎中次黄嘌呤(HX)的比色法灵敏监测。HX在黄嘌呤氧化酶(XOD)中存在时产生H_(2)O_(2)中间体,能够在Fe_(3)O_(4)@ZIF-8@HRP@SiO_(2)纳米酶复合体催化下氧化荧光红染料(AR),使溶液由无色变为红色,进而实现HX的定量分析。利用透射电镜(TEM),傅里叶变换红外光谱(FTIR),粒径分析等对材料形貌、元素组成、表面官能团进行表征。在最佳条件下,HX的检测范围为0.005~50μmol/L,检出限为0.00127μmol/L。与毛细管电泳法、电化学法等传统方法相比,线性范围更广,检测灵敏度更高。该方法被用于检测4℃和30℃连续存放96 h的牡蛎中HX含量的变化,验证了其用于实际样品检测的可行性。
In this study,we have constructed Fe_(3)O_(4)@ZIF-8@HRP@SiO_(2)nanocomposite materials,which improved the loading efficiency and stability of horseradish peroxidase(HRP),and used it for the sensitive colorimetric detection of hypoxanthine(HX)in oysters.As the product of the reaction between HX and xanthine oxidase(XOD),the H_(2)O_(2)intermediate can oxidize the Amplex red(AR),and form a red product under the catalysis of the Fe_(3)O_(4)@ZIF-8@HRP@SiO_(2)nanoenzyme complex,thus enabling the quantitative analysis of HX.The material's morphology,elemental composition,and surface functional groups were characterized using transmission electron microscopy(TEM),Fourier transform infrared spectroscopy(FTIR),and particle size analysis.Under the optimal conditions,the detection range for HX is 0.005-50μmol/L,with a limit of detection of 0.00127μmol/L.Compared with the traditional methods such as capillary electrophoresis and electrochemical methods,it has a wider linear range and higher detection sensitivity.This method was also used for detecting the HX content changes in oysters that were stored continuously for 96 hours at 4℃and 30℃,further verifying its feasibility for detecting actual samples.
作者
姚梦迪
高宇
孙舒扬
林立
张诺宣
王平
王承克
YAO Meng-di;GAO Yu;SUN Shu-yang;LIN Li;ZHANG Nuo-xuan;WANG Ping;WANG Cheng-ke(School of Food Engineering,Ludong University,Yantai 264025,China)
出处
《分析测试学报》
北大核心
2025年第10期2191-2197,共7页
Journal of Instrumental Analysis
基金
2021年山东省高等学校“青创人才引育计划”项目,山东省自然科学基金青年项目(ZR2023MC178)。
关键词
次黄嘌呤(HX)
辣根过氧化物酶
新鲜度
酶固定化
比色检测
hypoxanthine(HX)
horseradish peroxidase
freshness
enzyme immobilization
colorimetric determination
