摘要
该研究以肾上腺素和氯化锌为前驱体,采用水热法合成了一种新型的锌掺杂碳点(Zn/NA-CDs),考察了Zn/NA-CDs的光学性质,并结合X射线光电子能谱技术(XPS)、透射电子显微镜(TEM)、傅里叶红外光谱(FT-IR)和X射线衍射(XRD)等表征手段研究了Zn/NA-CDs的结构特征。Zn/NA-CDs具有较高的荧光发射效率,量子产率达43.28%。氨基酸与邻苯二甲醛(OPA)反应生成席夫碱产物,该产物可对Zn/NA-CDs产生荧光增敏作用。基于此原理,在优化条件下,以甘氨酸为模型分子构建了针对游离氨基酸的荧光检测体系,甘氨酸质量浓度在2.5~250 mg/L范围内,与体系的荧光强度呈线性关系,检出限为0.6 mg/L。该方法可用于实际烟叶样品中氨基酸的测定,实验结果令人满意。
In this study,a new type of zinc-doped carbon point(Zn/NA-CDs)was synthesized by hydrothermal method using epinephrine and zinc chloride as precursor.The optical properties of Zn/NA-CDs were studied by UV-Vis spectrophotometry and fluorescence spectrophotometry.The structural characteristics of Zn/NA-CDs were studied by X-ray photoelectron spectroscopy(XPS),transmission electron microscope(TEM),Fourier transform infrared spectroscopy(FT-IR),and X-ray diffraction(XRD).Zn/NA-CDs showed a relatively high photoluminescent quantum yield of about 43.28%.In this study,amino acids could react with phthalic aldehyde(OPA)to produce schiff base products,which can produce fluorescence sensitization on Zn/NA-CDs.Under the optimal analysis conditions,with glycine as the model molecule,the fluorescence intensity of the system was linear with the concentration of glycine in the range of 2.5-250 mg/L,and the detection limit was 0.6 mg/L.The method can be used for the determination of amino acids in actual tobacco samples,and satisfactory test results were obtained.
作者
何彬
周渭皓
高宪辉
张海
陈敬悦
王雨童
杨德志
杨亚玲
HE Bin;ZHOU Wei-hao;GAO Xian-hui;ZHANG Hai;CHEN Jing-yue;WANG Yu-tong;YANG De-zhi;YANG Ya-ling(Yunnan Tobacco Company,Kunming 650500,China;Faculty of Life Science and Technology,Kunming University of Technology,Kunming 650500,China)
出处
《分析测试学报》
北大核心
2025年第10期2177-2183,共7页
Journal of Instrumental Analysis
基金
中国烟草总公司云南省公司一般项目(2022530000216102)。
