摘要
目的探讨JMY和WHAMM两个自噬相关蛋白与胶质瘤自噬障碍及预后的相关性。方法收集弥漫性胶质瘤113例、对照脑组织14例,采用免疫组化EnVision两步法检测JMY、WHAMM、自噬标志物的表达;分析JMY、WHAMM表达与临床病理学特征、预后及自噬标志物表达的相关性。结果对照脑组织中JMY、WHAMM高表达率,明显高于弥漫性胶质瘤组织。JMY、WHAMM表达与病理分级、年龄相关,高级别胶质瘤(high-grade gliomas,HGG)与低级别胶质瘤(low-grade gliomas,LGG)相比,JMY、WHAMM高表达率明显较低。JMY、WHAMM低表达患者的总生存时间比高表达患者短。Cox回归分析显示:病理分级为高级别、JMY低表达是弥漫性胶质瘤患者预后的独立危险因素。自噬标志物的表达提示HGG自噬活性下降,JMY与自噬标志物的表达具有相关性。结论JMY、WHAMM在HGG中的高表达率明显低于LGG,可作为弥漫性胶质瘤预后评估的潜在分子标志物。
Purpose To investigate the correlation between two autophagy-related proteins,JMY and WHAMM,and autophagic dysfunction and prognosis in gliomas.Methods A total of 113 cases of diffuse gliomas and 14 control brain tissues were collected.The expression of JMY,WHAMM,and autophagy-related markers were evaluated using the EnVision two-step method of immunohistochemistry.Correlations between the expression of JMY and WHAMM and clinicopathological features,prognosis,and autophagy markers were analyzed.Results The high expression rates of JMY and WHAMM in control brain tissue were significantly higher than those in diffuse gliomas.The expressions of JMY and WHAMM were associated with pathological grade and age.Compared with low-grade gliomas(LGG),high-grade gliomas(HGG)showed significantly lower high-expression rates of JMY and WHAMM.Patients with low expression of JMY and WHAMM had shorter overall survival than those with high expression.Cox regression analysis indicated that the high pathological grade and low expression of JMY were independent prognostic risk factors.The expression profiles of autophagy markers suggested reduced autophagic activity in HGGs,and JMY expression was positively correlated with autophagy markers.Conclusion The high expression rates of JMY and WHAMM are significantly lower in HGGs compared to LGGs.JMY and WHAMM may serve as potential molecular markers for the prognostic evaluation of diffuse gliomas.
作者
范张伟
王雷明
熊艳蕾
高敏
胡泽良
赵莉红
滕梁红
Fan Zhangwei;Wang Leiming;Xiong Yanlei;Gao Min;Hu Zeliang;Zhao Lihong;Teng Lianghong(Department of Pathology,Xuanwu Hospital,Capital Medical University,Beijing 100053)
出处
《临床与实验病理学杂志》
北大核心
2025年第9期1142-1148,共7页
Chinese Journal of Clinical and Experimental Pathology
基金
京津冀专项项目[19JCZDJC64900(Z)]。
