摘要
采用连续流AOA反应器,通过接种污水处理厂生化池的活性污泥,并结合分段进水、缺氧池工作容积调整以及曝气量的精准调控等措施,以实现AOA系统的快速启动和性能优化。通过长期运行监测,全面探明污染物的长期去除性能以及典型运行周期内各污染物的变化情况。同时,运用高通量测序技术深入解析微生物群落结构组成,揭示功能菌群在不同运行参数下的变化规律。结果表明,分段进水可优化碳源利用路径,有利于内碳源储存;增加缺氧池工作容积,能够促进系统内源反硝化;提高曝气量,可保证好氧池硝化反应高效进行。最终系统平均出水COD、NH_(4)^(+)-N、TIN和PO_(4)^(3-)-P浓度分别为26.06、0.89、4.02和0.72 mg/L,去除率分别为92.99%、98.09%、91.53%和83.10%,且内碳源储存量与储存率分别达155.47 mg/L和97.3%。此外,污泥体积指数(SVI)稳定在115~125 mL/g,MLVSS/MLSS值升至0.66,系统污泥活性显著提升。高通量测序分析显示,氨氧化菌属Ellin6067与966-1的相对丰度保持稳定,Hyphomicrobium和Terrimonas等反硝化菌属的相对丰度由7.80%升高至14.06%,聚糖菌Candidatus_Competibacter相对丰度由0.66%提升至2.22%,这些功能菌共同保障了系统硝化反硝化脱氮性能,有效降低了出水TIN浓度。聚磷菌属Acinetobacter相对丰度也呈上升趋势,由0.08%提升至4.47%;反硝化聚磷菌Denitratisoma相对丰度由0.02%提高至0.77%,保证了系统除磷性能的稳定。
By inoculating activated sludge from the biochemical tank of the wastewater treatment plant,and combining measures such as step-feed,adjustment of the working volume of the anoxic tank,and precise control of the aeration rate,the rapid start-up and performance optimization of the AOA system were investigated.Long-term monitoring revealed the pollutants removal performance and the variation of each pollutant within a typical operational cycle.Using high-throughput sequencing technology,the microbial community structure was analyzed,and the variation patterns of functional bacterial communities under different operational parameters were identified.The results showed that step-feed could optimize carbon source utilization and benefit internal carbon storage.Increasing the anoxic zone’s working volume promoted endogenous denitrification in the system.Raising the aeration rate ensured efficient nitrification in the aerobic tank.The average effluent concentrations of COD,NH_(4)^(+)-N,TIN,and PO_(4)^(3-)-P were 26.06 mg/L,0.89 mg/L,4.02 mg/L,and 0.72 mg/L,respectively,with removal rates of 92.99%,98.09%,91.53%,and 83.10%.The internal carbon source storage and storage rate reached 155.47 mg/L and 97.3%,respectively.Additionally,the sludge volume index stabilized at 115-125 mL/g,and the MLVSS/MLSS ratio increased to 0.66,indicating significantly enhanced sludge activity.High-throughput sequencing analysis revealed that the relative abundance of ammonia-oxidizing bacterial genera Ellin6067 and 966-1 remained stable,while the relative abundance of denitrifying bacterial genera such as Hyphomicrobium and Terrimonas increased from 7.80%to 14.06%.The relative abundance of glycogen-accumulating bacterial genus Candidatus_Competibacter increased from 0.66%to 2.22%.These functional bacteria collectively ensured efficient nitrification and denitrification,effectively reducing the effluent TIN concentration.The relative abundance of phosphate-accumulating genus Acinetobacter increased from 0.08%to 4.47%,and that of the denitrifying phosphate-accumulating genus Denitratisoma increased from 0.02%to 0.77%,ensuring stable phosphorus removal performance.
作者
陈长乐
王继苗
陈群
黄青
赵骥
邱艳玲
冯娟
王晓霞
CHEN Chang-le;WANG Ji-miao;CHEN Qun;HUANG Qing;ZHAO Ji;QIU Yan-ling;FENG Juan;WANG Xiao-xia(School of Environment and Geography,Qingdao University,Qingdao 266071,China;Qingdao Water Group Co.Ltd.,Qingdao 266100,China;Qingdao Shuangyuan Water Group Co.Ltd.,Qingdao 266109,China)
出处
《中国给水排水》
北大核心
2025年第17期1-10,共10页
China Water & Wastewater
基金
国家自然科学基金资助项目(42477138、52070106)
山东省自然科学基金资助项目(ZR2023QE134)
山东省高等学校青创团队引育计划(城镇污水高效治理与资源化利用研究团队)项目。
关键词
城市污水
AOA工艺
分段进水
脱氮除磷
菌群结构
municipal wastewater
AOA process
step-feed
nitrogen and phosphorus removal
microbial community structure
