摘要
为探究2,4-表油菜素内酯(EBR)调控野艾蒿(Artemisia lavandulifolia)响应钼胁迫的作用机制,本研究采用盆栽试验,对2种浓度(250和500 mg·kg^(–1))钼处理下的野艾蒿进行叶片喷施EBR处理,分析EBR对钼胁迫下野艾蒿的缓解效应。研究结果表明,在未喷施EBR处理下,与土壤无钼处理和250 mg·kg^(–1)钼处理对比,高钼胁迫(500 mg·kg^(–1))对野艾蒿的生长生理过程产生强烈的不利影响,加速叶绿素降解,降低光合效率和抗氧化酶活性,加剧膜脂过氧化程度,破坏细胞渗透平衡,进而明显抑制植物生长和生物量的积累。外源喷施适宜浓度EBR(0.5 mg·L^(–1))处理能够显著缓解高钼胁迫的抑制作用,使野艾蒿株高、总根长、地上和地下生物量分别增加20.51%、51.65%、19.88%和39.18%,叶绿素含量和荧光强度升高,可溶性糖和可溶性蛋白含量分别增加30.54%和33.21%,超氧化物歧化酶、过氧化物酶和过氧化氢酶活性分别提高98.24%、69.23%和3.89%,同时显著降低了丙二醛(32.60%)和过氧化氢含量(41.18%)。结合相关性分析结果表明,EBR可通过提高光合能力、抗氧化酶活性和渗透调节物质水平的共同作用,有效增强野艾蒿对钼胁迫的耐受性,并缓解钼胁迫造成的氧化损伤和对野艾蒿生长的抑制作用。研究结果为揭示EBR缓解植物钼胁迫的生理机制提供了重要依据,可为EBR在钼矿区生态修复中的应用提供一定的理论指导。
In order to explore the mechanism of 2,4-epibrassinolide(EBR)in regulating molybdenum stress of Artemisia lavandulifolia,a pot experiment was conducted to analyse the ameliorative effect of foliar-applied EBR on A.lavandulifolia under two levels of molybdenum stress(250 and 500 mg.kg*1 Mo).The results showed that under unsprayed EBR treatment,compared with the soil Mo-free treatment and 250 mg-kg1 Mo treatment,high molybdenum stress had a strong adverse effect on the growth and physiological process of A.lavandulifolia.High molybdenum stress(500 mg·kg^(-1))accelerated the decomposition of chlorophyll,reduced photosynthetic efficiency and antioxidant enzyme activity,aggravated the degree of membrane lipid peroxidation,destroyed cellular osmotic balance,and thus significantly inhibited plant growth and biomass accumulation.Exogenous EBR(0.5 mg·L^(-1))treatment significantly alleviated the negative effects of molybdenum stress(500 mg·kg^(-1)),increasing plant height,total root length,aboveground and underground biomass of A.lavandulifolia by 20.51%,51.65%,19.88%and 39.18%,the chlorophyll content and fluorescence intensity,soluble sugar and soluble protein contents by 30.54%and 33.21%,and enhancing superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities by 98.24%,69.23%and 3.89%,respectively,while significantly reducing malondialdehyde(MDA)and hydrogen peroxide(H,02)contents by 32.60%and 41.18%.The results of the combined correlation analysis indicate that EBR can effectively enhance the tolerance of A.lavandulifolia to molybdenum stress,alleviate the oxidative damage caused by molybdenum stress and the inhibitory effect on the growth of A.lavandulifolia through the combined action of improving photosynthetic capacity,antioxidant enzyme activities,and osmoregulatory substance levels.The results provide an important basis for revealing the physiological mechanisms of EBR in alleviating plant molybdenum stress and can provide some theoretical guidance for potential applications of EBR in ecological restoration of molybdenum contaminated mining areas.
作者
靳祖璇
马思祖
李亚鸽
赵威
JIN Zuxuan;MA Sizu;LI Yage;ZHAO Wei(College of Agriculture,Henan University of Science and Technology,Luoyang,Henan 471000,China)
出处
《植物生理学报》
北大核心
2025年第8期1180-1190,共11页
Plant Physiology Journal
基金
国家自然科学基金委员会-河南省人民政府人才培养联合基金项目(U1304306)。
关键词
2
4-表油菜素内酯
野艾蒿
钼胁迫
生理机制
2,4-epibrassinolide(EBR)
Artemisia lavandulifolia
molybdenum stress
physiological mechanism
