摘要
病程相关蛋白1(pathogenesis-related proteins 1,PR1)在植物抵御胁迫及生长发育中有着重要的作用。本研究通过生物信息学手段系统分析鉴定了8个白木香As PR1基因,预测其编码蛋白的分子质量在12.95~43.41 k D之间。蛋白的二维结构及保守基序分析表明,8个As PR1蛋白序列的相似度为51.38%,都具有保守的CAP-PR1(cysteine-rich secretory proteins,CAP)结构域。顺式作用元件分析表明,As PR1基因家族具有响应植物激素、非生物胁迫和生物进程相关的顺式作用元件。染色体定位显示As PR1基因分布在1、3、4、8号染色体上。根据转录组分析数据本研究从白木香中克隆得到As PR1-4序列,全长为516 bp,编码171个氨基酸;成功进行As PR1-4-GST融合蛋白表达。亚细胞定位实验结果表明As PR1-4定位于细胞质和叶绿体中。实时荧光定量PCR结果表明,As PR1-4在茎中表达量最高,其次是根,叶中最少;茉莉酸甲酯(methyl jasmonate,Me JA)和水杨酸(salicylic acid,SA)诱导下As PR1-4表达量均有不同程度的增加;而As PR1-4在盐、低温、重金属和干旱四种非生物胁迫下表达量均显著下降。本研究为进一步揭示PR1蛋白在白木香防御反应中的功能及其在沉香生产中的应用奠定基础。
Pathogenesis related protein 1(PR1)plays an important role in plant resistance to stress and growth and development.This study identified and systematically analyzed 8 AsPR1 genes in Aquilaria sinensis using bioinformatics methods.The predicted molecular weight of encoded AsPR1 proteins is between 12.95 kD and 43.41 kD.The two-dimensional structure and conserved motif analysis of the AsPR1 proteins demonstrated that the similarity of 8 AsPR1 proteins was 51.38%,and all 8 AsPR1 proteins had conserved CAP-PR1 domains.Cis-acting elements analysis showed that the AsPR1 gene family proteins have cis-acting elements that respond to plant hormones,abiotic stress,and biological processes.Chromosomal mapping showed that the AsPR1 gene was distributed on chromosomes 1,3,4 and 8.The full-length AsPR1-4 sequence was successfully cloned according to the transcrip tome sequencing analysis from A.sinensis,which contains a 516 bp ORF that encodes 171 amino acids.Further more,we successfully expressed AsPR1-4-GST fusion protein.The subcellular localization experiments indicated that AsPR1-4 is located in the cytoplasm and chloroplast.The real-time fluorescence quantitative PCR results showed that AsPR1-4 had the highest expression level in stems,followed by roots,and the lowest expression level in leaves.Exogenous MeJA and SA significantly increased the expression of AsPR1-4 in Calli.Additionally,the transcripts level of AsPR1-4 remarkably decreased under four abiotic stresses,including salt,low temperature,heavy metals,and drought stress.This study lays the foundation for further elucidating the function of PR1 protein in the defense response of A.sinensis and its application in agarwood production.
作者
郑语嫣
关范圆
范菁
邱海玲
米芯雨
王宝纬
高博闻
王晓晖
ZHENG Yu-yan;GUAN Fan-yuan;FAN Jing;QIU Hai-ling;MI Xin-yu;WANG Bao-wei;GAO Bo-wen;WANG Xiao-hui(Modern Research Center for Traditional Chinese Medicine,Beijing Institute of Traditional Chinese Medicine,Beijing University of Chinese Medicine,Beijing 102488,China;School of Chinese Pharmacy,Beijing University of Chinese Medicine,Beijing 102488,China;School of Pharmacy,Baotou Medical College,Baotou 014040,China)
出处
《药学学报》
北大核心
2025年第9期2890-2900,共11页
Acta Pharmaceutica Sinica
基金
国家自然科学基金面上项目(32170374)
内蒙古自然科学基金(2023MS08011)。
关键词
白木香
病程相关蛋白1
生物信息学
非生物胁迫
表达分析
Aquilaria sinensis
pathogenesis related protein 1
bioinformatics
abiotic stress
expression analysis
